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Home >> Antibodies >> YY1 Antibody / Ying-Yang 1

YY1 Antibody / Ying-Yang 1 [clone YY1/5436] (V4366)

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  Catalog No Formulation Size Price (USD)  
Image V4366-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4366-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4366SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of human Daudi cell lysate with YY1 Mouse Monoclonal (YY1/5436). Predicted molecular weight ~45 kDa but commonly observed at 45~65 kDa.
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Western blot testing of human Daudi cell lysate with YY1 antibody (YY1/5436). Predicted molecular weight ~45 kDa but commonly observed at 45~65 kDa.
IHC staining of FFPE human tonsil tissue with YY1 antibody (YY1/5436). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free YY1 antibody (clone YY1/5436) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2, kappa
Clone Name YY1/5436
Purity Protein A/G affinity
UniProt P25490
Localization Nucleus
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This YY1 antibody is available for research use only.
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Description

The YY1 transcription factor, also known as NF-E1 (human) and Delta or UCRBP (mouse) is of interest due to its diverse effects on a wide variety of target genes. YY1 is broadly expressed in a wide range of cell types and contains four C-terminal zinc finger motifs of the Cys-Cys-His-His type and an unusual set of structural motifs at its N-terminal end. It binds to downstream elements in several vertebrate ribosomal protein genes, where it apparently acts positively to stimulate transcription and can act either negatively or positively in the context of the immunoglobulin k 3' enhancer and immunoglobulin heavy-chain �E1 site as well as the P5 promoter of the adenoassociated virus. It thus appears that YY1 is a bifunctional protein, capable of functioning as an activator in some transcriptional control elements and a repressor in others.

Application Notes

Optimal dilution of the YY1 antibody should be determined by the researcher.

Immunogen

A His-tagged YY1 protein was used as the immunogen for the YY1 antibody.

Storage

Aliquot the YY1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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