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Home >> Antibodies >> YY1 Antibody / Yin and Yang 1

YY1 Antibody / Yin and Yang 1 (F48079)

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  Catalog No Formulation Size Price (USD)  
Image F48079-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 429
Image
F48079-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 199
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Western blot testing of human MOLT4 cell lysate with YY1 antibody. Predicted molecular weight ~45 kDa but commonly observed at 45~65 kDa.
Western blot testing of human MOLT4 cell lysate with YY1 antibody. Predicted molecular weight ~45 kDa but commonly observed at 45~65 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P25490
Applications Western Blot : 1:1000-1:2000
Limitations This YY1 antibody is available for research use only.
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Description

Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements. [UniProt]

Application Notes

Titration of the YY1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 268-297 from the human protein was used as the immunogen for this YY1 antibody.

Storage

Aliquot the YY1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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