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Home >> Antibodies >> YAP Antibody

YAP Antibody (F49693)

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  Catalog No Formulation Size Price (USD)  
Image F49693-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F49693-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of YAP antibody and NCI-H460 lysate. Predicted molecular weight ~54 kDa but routinely observed at 65-70 kDa.
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Western blot analysis of YAP antibody and NCI-H460 lysate. Predicted molecular weight ~54 kDa but routinely observed at 65-70 kDa.
YAP antibody flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P46937
Applications Western blot : 1:1000
Flow Cytometry : 1:10-1:50
Limitations This YAP antibody is available for research use only.
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Description

YAP is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. [UniProt]

Application Notes

Titration of the YAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 420-446 from human YAP1 was used as the immunogen for this YAP antibody.

Storage

Aliquot the YAP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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