- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
XRCC5 Antibody / Ku Heterodimer DNA End-Binding Antibody [clone XRCC5/7316] (V4940)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4940-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V4940-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V4940SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1 + IgG2b, kappa |
| Clone Name | XRCC5/7316 |
| Purity | Protein A/G affinity |
| UniProt | P13010 |
| Localization | Nucleus |
| Applications | Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This XRCC5 antibody is available for research use only. |
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X-ray repair cross-complementing protein 5 (XRCC5), also known as Ku80, is a DNA end-binding protein that functions as part of the Ku heterodimer together with XRCC6 (Ku70). XRCC5 Antibody is uniquely positioned for studies focused on DNA end recognition, where the ability of proteins to bind exposed DNA termini is critical for initiating repair of double-strand breaks. The Ku heterodimer is one of the first protein complexes to engage broken DNA, forming a stable ring-like structure that encircles DNA ends and protects them from degradation.
XRCC5 Antibody is uniquely positioned for investigating the molecular mechanics of DNA end-binding, a defining feature that distinguishes the Ku complex from other DNA repair proteins. XRCC5 antibody, also referred to as Ku80 antibody, detects a nuclear protein that exhibits high affinity for DNA termini regardless of sequence context. This sequence-independent binding allows XRCC5 to act as a universal sensor of DNA double-strand breaks, making it essential for rapid and efficient detection of genomic damage.
The DNA end-binding activity of XRCC5 enables recruitment of additional repair factors, including DNA-dependent protein kinase catalytic subunit and ligation enzymes that complete the non-homologous end joining process. Without this initial binding step, repair complexes cannot assemble properly, leading to accumulation of DNA damage and compromised genomic stability. Detection of XRCC5 therefore provides direct insight into the earliest stages of DNA damage recognition and repair initiation.
XRCC5 is broadly expressed across cell types and is particularly active in proliferating cells, where DNA replication increases the likelihood of double-strand breaks. Its DNA-binding function can be influenced by chromatin accessibility, DNA damage load, and cellular stress conditions, making it a valuable marker for studying DNA-protein interactions under a wide range of biological contexts. In experimental systems, XRCC5 detection can be used to assess the presence of DNA ends and the efficiency of repair initiation.
As a member of the DNA repair protein family, XRCC5 integrates structural DNA binding with recruitment of repair machinery, serving as a bridge between damage detection and repair execution. Its role as a DNA end-binding component of the Ku heterodimer provides a distinct functional identity that is not shared by downstream repair enzymes. XRCC5 Antibody provides a focused tool for researchers studying DNA end recognition, repair complex assembly, and the molecular architecture of DNA repair pathways.
Optimal dilution of the XRCC5 Antibody / Ku Heterodimer DNA End-Binding Antibody should be determined by the researcher.
A recombinant partial protein sequence (within amino acids 300-500) from the human protein was used as the immunogen for the XRCC5 Antibody / Ku Heterodimer DNA End-Binding Antibody.
Aliquot the XRCC5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Ku80 antibody, XRCC5 Ku heterodimer antibody, Ku80 DNA end-binding antibody, XRCC5 DNA binding protein antibody, Ku complex antibody
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