XPO1 Antibody / Exportin 1 / CRM1 [clone 5G3] (RQ4517)

Western blot testing of 1) rat liver, 2) rat lung, 3) mouse liver, 4) mouse lung, 5) rabbit IgG, 6) molecular weight marker, 7) human HepG2, 8) human SMMC-7721, 9) human HeLa and 10) human Jurkat lysate with XPO1 antibody at 0.5ug/ml. Expected molecular weight ~123 kDa.

IHC testing of FFPE human lung cancer with XPO1 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human intestinal cancer with XPO1 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry testing of human SiHa cells with XPO1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= XPO1 antibody.

Immunofluorescent staining of FFPE human U-2 OS cells with XPO1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.