WWTR1 Antibody / Hippo Pathway Effector Antibody (FY13463)

WWTR1 Antibody / Hippo Pathway Effector Antibody Human Ovarian Cancer IHC. Immunohistochemical staining of FFPE human ovarian cancer tissue using WWTR1 Antibody / Hippo Pathway Effector Antibody. WWTR1 expression is detected predominantly within the nuclei and cytoplasm of tumor cells, consistent with its role as a Hippo pathway-regulated transcriptional coactivator that controls cell proliferation, survival, and epithelial-to-mesenchymal transition. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

WWTR1 Antibody / Hippo Pathway Effector Antibody Ovarian Adenocarcinoma IHC. Immunohistochemical staining of FFPE human ovarian adenocarcinoma tissue using WWTR1 Antibody / Hippo Pathway Effector Antibody. WWTR1 expression is detected predominantly within the nuclei and cytoplasm of neoplastic epithelial cells, consistent with its role as a Hippo pathway-regulated transcriptional coactivator involved in cell proliferation, survival, and epithelial-to-mesenchymal transition. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

WWTR1 Antibody / Hippo Pathway Effector Antibody Mouse Brain IHC. Immunohistochemical staining of FFPE mouse brain tissue using WWTR1 Antibody / Hippo Pathway Effector Antibody. WWTR1 expression is detected predominantly within the nuclei of neuronal cells, consistent with its role as a Hippo pathway-regulated transcriptional coactivator involved in neural development, cell fate determination, and transcriptional control. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

WWTR1 Antibody / Hippo Pathway Effector Antibody Rat Brain IHC. Immunohistochemical staining of FFPE rat brain tissue using WWTR1 Antibody / Hippo Pathway Effector Antibody. WWTR1 expression is detected predominantly within the nuclei of neuronal cells, consistent with its role as a Hippo pathway-regulated transcriptional coactivator involved in neural development, cell fate determination, and transcriptional regulation. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

WWTR1 Antibody / Hippo Pathway Effector Antibody Rat Kidney IHC. Immunohistochemical staining of FFPE rat kidney tissue using WWTR1 Antibody / Hippo Pathway Effector Antibody. WWTR1 expression is detected predominantly within the nuclei of renal tubular epithelial cells, consistent with its role as a Hippo pathway-regulated transcriptional coactivator involved in cell proliferation, tissue homeostasis, and transcriptional regulation. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

WWTR1 Antibody / Hippo Pathway Effector Antibody U251 Cell IF. Immunofluorescent staining of U251 cells using WWTR1 Antibody / Hippo Pathway Effector Antibody. WWTR1 is detected predominantly within the nuclei (green), consistent with its role as a Hippo pathway-regulated transcriptional coactivator that undergoes nuclear localization during transcriptional activation. Alpha Tubulin (red) was used as a cytoskeletal marker. Enzymatic antigen retrieval was performed for 15 minutes prior to staining. Cells were blocked with 10% goat serum and incubated overnight at 4°C with rabbit anti-WWTR1 antibody (5 ug/ml) and mouse anti-Alpha Tubulin antibody, followed by Fluoro488-conjugated goat anti-rabbit IgG and Cy3-conjugated goat anti-mouse IgG secondary antibodies. Fluorescence was visualized using appropriate filter sets.

WWTR1 Antibody / Hippo Pathway Effector Antibody Cancer Cell Line WB. Western blot analysis of 1) human A431, 2) human U251, and 3) human A549 whole cell lysates using WWTR1 Antibody / Hippo Pathway Effector Antibody. A specific immunoreactive band is detected at approximately 50 kDa in all three cell lines, consistent with WWTR1 protein expression. The predicted molecular weight of WWTR1 is approximately 44 kDa, with the observed migration likely reflecting post-translational modification or altered electrophoretic mobility.

WWTR1 Antibody / Hippo Pathway Effector Antibody Immunoprecipitation Validation. Immunoprecipitation of WWTR1 from HeLa whole cell lysate using WWTR1 Antibody / Hippo Pathway Effector Antibody, followed by western blot detection with the same antibody. Lane 1: Input HeLa whole cell lysate. Lane 2: Rabbit control IgG immunoprecipitation (negative control). Lane 3: WWTR1 antibody immunoprecipitation. A specific band is detected at approximately 50 kDa in the input and WWTR1 immunoprecipitated sample, while no corresponding band is observed in the control IgG lane, confirming specific enrichment of WWTR1. The predicted molecular weight of WWTR1 is approximately 44 kDa.