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Home >> Antibodies >> WNT5B Antibody

WNT5B Antibody (RQ5929)

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  Catalog No Formulation Size Price (USD)  
Image RQ5929 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human U-87 MG cells with WNT5B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WNT5B antibody.
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Flow cytometry testing of human U-87 MG cells with WNT5B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WNT5B antibody.
Western blot testing of human 1) HEK293 and 2) SW620 lysate with WNT5B antibody. Expected molecular weight: 40-45 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q9H1J7
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This WNT5B antibody is available for research use only.
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    Reactivity : Human
  • Applications : WB, IHC, FACS, ELISA
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    Pred. Reactivity : Rat, Rabbit, Xenopus

Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants.

Application Notes

Optimal dilution of the WNT5B antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids Q18-K218) was used as the immunogen for the WNT5B antibody.

Storage

After reconstitution, the WNT5B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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