WNT4 Antibody / Developmental Signaling Protein Antibody (RQ5232)

WNT4 Antibody Human Kidney IHC. Immunohistochemical analysis of FFPE human kidney tissue using WNT4 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody at 2 ug/mL overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Strong cytoplasmic staining is observed throughout the renal tubular epithelium, while glomeruli exhibit comparatively weaker staining, consistent with endogenous WNT4 expression in kidney tissue. These results demonstrate that WNT4 Antibody, a Developmental Signaling Protein Antibody, specifically detects endogenous WNT4 in FFPE human tissue.

WNT4 Antibody Mouse Kidney IHC. Immunohistochemical analysis of FFPE mouse kidney tissue using WNT4 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody at 2 ug/mL overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Prominent cytoplasmic staining is observed throughout the renal tubular epithelium, while glomeruli show comparatively weaker immunoreactivity, consistent with endogenous WNT4 expression in mouse kidney. These results demonstrate that WNT4 Antibody, a Developmental Signaling Protein Antibody, specifically detects endogenous WNT4 in FFPE mouse tissue.

WNT4 Antibody Rat Kidney IHC. Immunohistochemical analysis of FFPE rat kidney tissue using WNT4 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody at 2 ug/mL overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate to strong cytoplasmic staining is observed throughout the renal tubular epithelium, with comparatively weaker staining in the glomeruli, consistent with endogenous WNT4 expression in rat kidney. These results demonstrate that WNT4 Antibody, a Developmental Signaling Protein Antibody, specifically detects endogenous WNT4 in FFPE rat tissue.

WNT4 Antibody Human, Rat and Mouse WB. Western blot analysis of WNT4 Antibody using lysates from 1) human MCF-7 cells, 2) human HeLa cells, 3) human Jurkat cells, 4) human K562 cells, 5) rat testis tissue, and 6) mouse testis tissue. Samples (30 ug/lane) were separated on a 10% SDS-PAGE gel under reducing conditions, transferred to a nitrocellulose membrane, and detected with WNT4 Antibody at 0.5 ug/mL followed by an HRP-conjugated secondary antibody and ECL detection. A specific band is observed at approximately 45-50 kDa in all samples, migrating above the predicted molecular weight of approximately 39 kDa, likely reflecting post-translational modification such as glycosylation, which is common for secreted Wnt proteins. These results demonstrate that WNT4 Antibody, a Developmental Signaling Protein Antibody, specifically detects endogenous WNT4 across human, rat, and mouse samples.