vWF Antibody Clone VWF/1465 / von Willebrand Factor Antibody [clone VWF/1465] (V3417)

vWF Antibody Clone VWF/1465 western blot analysis. Western blot of 1) partial recombinant protein and 2) human lung lysate using vWF Antibody Clone VWF/1465 (clone VWF/1465). Lane 1 shows a lower molecular weight band corresponding to a truncated recombinant fragment, while lane 2 shows a prominent band at approximately 250 kDa, consistent with the predicted molecular weight of von Willebrand factor (VWF). The higher molecular weight signal in lung lysate is consistent with the heavily glycosylated nature of VWF, which undergoes extensive post-translational modification and multimerization, often producing an elevated or broadened apparent molecular weight on SDS-PAGE relative to the core protein.

IHC testing of FFPE human tonsil with vWF antibody (clone VWF/1465). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

vWF Antibody Clone VWF/1465 / von Willebrand factor Antibody microarray specificity validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using vWF Antibody Clone VWF/1465 demonstrates strong and selective binding to VWF, supporting high target specificity. The top-ranked signal corresponds to von Willebrand factor, with substantially lower signals observed for non-target proteins, indicating minimal cross-reactivity. Z-score represents the strength of antibody binding signal relative to the mean signal across the array, expressed in standard deviations. S-score reflects the difference between successive Z-scores and provides a measure of relative specificity for the intended target.

SDS-PAGE analysis of purified, BSA-free vWF antibody (clone VWF/1465) as confirmation of integrity and purity.