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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
vWF Antibody for FACS / von Willebrand Factor Flow Cytometry Antibody [clone 907CT12.1.9] (F52535)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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F52535-0.4ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.4 ml | 439 | |
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F52535-0.08ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.08 ml | 205 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1 |
| Clone Name | 907CT12.1.9 |
| Purity | Purified |
| UniProt | P04275 |
| Applications | IHC (Paraffin) : 1:25 Flow Cytometry : 1:100 Western Blot : 1:1000 |
| Limitations | This vWF antibody is available for research use only. |
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Von Willebrand factor (VWF) is a large secreted glycoprotein encoded by the VWF gene and synthesized primarily by vascular endothelial cells and megakaryocytes, where it is stored in intracellular secretory granules. vWF Antibody for FACS is designed for flow cytometry applications requiring intracellular detection of VWF, enabling identification and characterization of VWF-positive cell populations at the single-cell level. VWF antibody, also referred to as von Willebrand factor antibody, is widely used as a marker of endothelial and megakaryocytic lineages in flow cytometry-based analyses.
In flow cytometry, VWF detection depends on fixation and permeabilization, as the protein is localized within Weibel-Palade bodies in endothelial cells and alpha granules in megakaryocytes rather than on the cell surface. This intracellular localization produces a distinct staining profile that allows clear separation of VWF-positive and VWF-negative populations. As a result, VWF antibody is particularly useful for distinguishing endothelial-derived cells and megakaryocytic cells within heterogeneous samples.
vWF Antibody for FACS is optimized for applications where signal clarity and population resolution are critical for accurate gating and analysis. Clone 907CT12.1.9 provides consistent intracellular staining, enabling reliable identification of VWF-expressing cells even when present at low frequency. This supports precise discrimination of positive populations in complex samples such as mixed cell suspensions, primary tissues, or cultured systems.
In multiparametric flow cytometry, VWF antibody is often used alongside surface markers to define endothelial lineage or platelet-producing cell populations with greater specificity. Its intracellular signal complements membrane marker panels, allowing researchers to refine gating strategies and improve confidence in population identification. This is particularly valuable in studies of vascular biology, hematopoiesis, and disease-associated changes in endothelial or megakaryocytic compartments.
Because VWF is not typically expressed on the cell surface, intracellular staining is essential for accurate detection in flow cytometry. vWF Antibody for FACS supports these analyses by enabling clear identification of VWF-positive populations, making it well suited for studies focused on endothelial cell characterization, megakaryocyte biology, and single-cell analysis of vascular-associated lineages.
Titration of the vWF Antibody for FACS / von Willebrand Factor Flow Cytometry Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
This vWF Antibody for FACS / von Willebrand Factor Flow Cytometry Antibody was produced from a mouse immunized with human recombinant protein.
Aliquot the vWF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
von Willebrand factor FACS antibody, VWF flow cytometry antibody, von Willebrand factor clone 907CT12.1.9 antibody, VWF clone 907CT12.1.9 antibody, endothelial marker VWF FACS antibody
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