- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
von Willebrand Factor Antibody / Megakaryocyte Lineage Marker Antibody [clone WFA52-2] (V7082)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V7082-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
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V7082-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
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V7082SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | WFA52-2 |
| Purity | Protein G purified |
| Gene ID | 7450 |
| Localization | Cytoplasmic |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This von Willebrand Factor antibody is available for research use only. |
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Von Willebrand factor (VWF) is a large secreted glycoprotein encoded by the VWF gene and synthesized primarily by vascular endothelial cells and megakaryocytes. von Willebrand Factor Antibody / Megakaryocyte Lineage Marker Antibody recognizes a protein that is actively produced within platelet-forming cells, making it highly relevant for identifying megakaryocytic lineage populations in hematopoietic tissue. VWF antibody, also referred to as von Willebrand factor antibody or factor VIII-related antigen antibody, is widely used in marrow-based studies where distinguishing platelet-producing cells from other hematopoietic lineages is essential.
Megakaryocytes are large, specialized cells responsible for platelet production within the bone marrow. These cells undergo a unique maturation process characterized by polyploidization, cytoplasmic expansion, and proplatelet formation. During this differentiation program, VWF is synthesized and incorporated into platelet precursor structures, linking megakaryocyte biology directly to hemostatic function. von Willebrand Factor Antibody is therefore particularly useful for detecting megakaryocytes and related lineage cells within complex marrow environments.
In bone marrow tissue, megakaryocytes are interspersed among diverse hematopoietic populations, including erythroid, myeloid, and lymphoid cells. While their large size and multilobulated nuclei provide morphological clues, protein-level markers such as VWF add an additional layer of specificity for lineage identification. VWF expression within these cells allows clear distinction of megakaryocytic populations from surrounding marrow elements, supporting accurate interpretation of cellular composition and lineage distribution.
VWF produced in megakaryocytes is packaged into platelet alpha-granules and contributes to the functional properties of circulating platelets. This establishes a direct connection between megakaryocyte differentiation and platelet-mediated hemostasis. Detection of VWF in these cells therefore reflects not only lineage identity but also the molecular preparation of platelets for adhesive and coagulation-related functions. This dual relevance strengthens its value in studies examining platelet biogenesis and hematopoietic specialization.
In experimental systems, VWF staining within marrow or cultured hematopoietic cells can reveal the presence, distribution, and relative abundance of megakaryocytic populations. This is particularly useful in studies of thrombopoiesis, lineage commitment, and hematopoietic differentiation pathways. Because VWF is not broadly expressed across all marrow cell types, its detection provides a focused readout of platelet-producing compartments within heterogeneous cellular environments.
von Willebrand Factor Antibody / Megakaryocyte Lineage Marker Antibody is especially suited for research centered on megakaryocyte identification, platelet lineage biology, and bone marrow cell characterization. By highlighting a protein that is integral to platelet-producing cells, it enables clear visualization of megakaryocytic populations and supports detailed analysis of hematopoietic lineage structure and function.
Variations in protocols, secondaries and substrates may require the von Willebrand Factor Antibody / Megakaryocyte Lineage Marker Antibody to be titered for optimal performance.
A recombinant human protein fragment was used as the immunogen for this von Willebrand Factor Antibody / Megakaryocyte Lineage Marker Antibody.
Store the von Willebrand Factor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
VWF megakaryocyte marker antibody, von Willebrand factor platelet lineage antibody, megakaryocytic marker antibody, VWF marrow megakaryocyte antibody, platelet producing cell marker antibody
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