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Home >> Antibodies >> von Willebrand Factor Antibody / vWF

von Willebrand Factor Antibody / vWF [clone VWF/4106] (V8821)

  Catalog No Formulation Size Price (USD)  
Image V8821-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8821-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8821SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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IHC staining of FFPE human tonsil tissue with von Willebrand Factor antibody (clone VWF/4106). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic tissue with von Willebrand Factor antibody (clone VWF/4106). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free von Willebrand Factor antibody (VWF/4106) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using von Willebrand Factor antibody (clone VWF/4106). These results demonstrate the foremost specificity of the VWF/4106 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name VWF/4106
Purity Protein A/G affinity
UniProt P04275
Localization Secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This von Willebrand Factor antibody is available for research use only.
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Description

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Application Notes

Optimal dilution of the von Willebrand Factor antibody should be determined by the researcher.

Immunogen

A portion of amino acids 1800-2000 was used as the immunogen for the von Willebrand Factor antibody.

Storage

Aliquot the von Willebrand Factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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