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Home >> Antibodies >> von Willebrand Factor Antibody

von Willebrand Factor Antibody [clone VWF/4105] (V4245)

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  Catalog No Formulation Size Price (USD)  
Image V4245-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4245-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4245SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE mouse lung with vWF antibody (clone VWF/4105). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse lung with vWF antibody (clone VWF/4105). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat lung with vWF antibody (clone VWF/4105). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human uterus tissue with vWF antibody (clone VWF/4105). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free vWF antibody (clone VWF/4105) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name VWF/4105
Purity Protein A/G affinity
UniProt P04275
Localization Secreted, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This von Willebrand Factor antibody is available for research use only.
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Description

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Application Notes

Optimal dilution of the von Willebrand Factor antibody should be determined by the researcher.

Immunogen

A recombinant human vWF fragment protein sequence (within amino acids 845-949) was used as the immunogen for the von Willebrand Factor antibody.

Storage

Aliquot the von Willebrand Factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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