VCL Antibody / Vinculin Antibody [clone VCL/3617] (V8475)

VCL Antibody Human U-87 MG Cell IF. Immunofluorescence analysis of PFA-fixed human U-87 MG cells demonstrates Vinculin expression (green) using VCL Antibody / Vinculin Antibody, clone VCL/3617, with nuclei counterstained using RedDot (red). The staining reveals predominantly cytoplasmic localization with enrichment at sites of cell adhesion, consistent with the known distribution of vinculin within focal adhesion complexes. This VCL Antibody is well suited for investigating focal adhesion dynamics, cytoskeletal organization, cell adhesion, and mechanotransduction signaling.

VCL Antibody Human Testis IHC. Immunohistochemical analysis of FFPE human testis demonstrates strong Vinculin expression with prominent membranous and cytoplasmic staining in seminiferous tubules. Heat-induced epitope retrieval was performed in pH9 Tris-EDTA buffer before staining with VCL Antibody / Vinculin Antibody, clone VCL/3617, followed by HRP detection and DAB visualization. The staining pattern is consistent with the localization of vinculin at focal adhesion complexes and sites of cytoskeletal attachment. This VCL Antibody is well suited for studies of cell adhesion, focal adhesion biology, cytoskeletal organization, and VCL protein expression.

VCL Antibody Human Testis Seminiferous Tubule IHC. Immunohistochemical analysis of FFPE human testis demonstrates strong Vinculin expression with prominent membranous and cytoplasmic staining throughout the seminiferous tubules. Heat-induced epitope retrieval was performed in pH9 Tris-EDTA buffer before staining with VCL Antibody / Vinculin Antibody, clone VCL/3617, followed by HRP detection and DAB visualization. The distinct staining pattern highlights Vinculin localization at sites of cell-cell and cell-matrix adhesion within the seminiferous epithelium. This VCL Antibody is well suited for studies of focal adhesion biology, cytoskeletal organization, cell adhesion, and VCL protein expression.

VCL Antibody Human U-87 MG FACS. Flow cytometry analysis of PFA-fixed human U-87 MG cells stained with VCL Antibody / Vinculin Antibody, clone VCL/3617, shows a pronounced rightward fluorescence shift (blue) compared with the isotype control (red), demonstrating specific detection of intracellular Vinculin. The clear separation between the antibody and control populations supports selective staining of VCL in U-87 MG cells. This VCL Antibody is suitable for studies of focal adhesion biology, cytoskeletal organization, cell adhesion, and mechanotransduction signaling.

VCL Antibody HuProt Protein Microarray Validation. Protein microarray analysis using a HuProt(TM) array containing more than 19,000 full-length human proteins demonstrates the exceptional specificity of VCL Antibody / Vinculin Antibody, clone VCL/3617. VCL was identified as the top-ranked target with a Z-score of 133.38 and an S-score of 123.59, while all other proteins produced substantially weaker signals. These results confirm highly selective recognition of vinculin with minimal off-target binding, supporting the use of this clone for studies of focal adhesion biology, cytoskeletal organization, cell adhesion, and mechanotransduction signaling.

SDS-PAGE analysis of purified, BSA-free Vinculin antibody as confirmation of integrity and purity.