Vinculin Antibody [clone VCL/3617] (V8475)

Immunofluorescent staining of PFA-fixed human U-87 MG cells with Vinculin antibody (clone VCL/3617, green) and Reddot nuclear stain (red).

IHC staining of FFPE human testis with Vinculin antibody (clone VCL/3617). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human testis with Vinculin antibody (clone VCL/3617). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry testing of PFA-fixed human U-87 MG cells with Vinculin antibody (clone VCL/3617); Red=isotype control, Blue= Vinculin antibody.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Vinculin antibody (clone VCL/3617). These results demonstrate the foremost specificity of the VCL/3617 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Vinculin antibody as confirmation of integrity and purity.