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Home >> Antibodies >> Vinculin Antibody

Vinculin Antibody [clone rVCL/7287] (V4103)

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  Catalog No Formulation Size Price (USD)  
Image V4103-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4103-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4103SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human lung tissue with Vinculin antibody (clone rVCL/7287). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung tissue with Vinculin antibody (clone rVCL/7287). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rVCL/7287
Purity Protein A/G affinity
UniProt P18206
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This Vinculin antibody is available for research use only.
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Description

Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and Alpha-Actinin. In addition, vinculin, Talin and Alpha-Actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. Alpha-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

Application Notes

Optimal dilution of the Vinculin antibody should be determined by the researcher.

Immunogen

A recombinant fragment of human Vinculin (VCL) protein was used as the immunogen for the Vinculin antibody.

Storage

Aliquot the Vinculin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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