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Home >> Antibodies >> Vinculin Antibody

Vinculin Antibody (F54510)

  Catalog No Formulation Size Price (USD)  
Image F54510-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
Image
F54510-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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Flow cytometry testing of fixed and permeabilized mouse NIH 3T3 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.
Flow cytometry testing of fixed and permeabilized mouse C2C12 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.
Western blot testing of 1) human skeletal muscle, 2) human A431, 3) human HeLa, 4) mouse kidney, 5) rat PC-12 and 6) human HepG2 cell lysate with Vinculin antibody. Predicted molecular weight ~124 kDa.
IHC testing of FFPE mouse skeletal muscle tissue with Vinculin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q64727
Applications Western blot : 1:500-1:2000
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This Vinculin antibody is available for research use only.
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Description

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).

Application Notes

The stated application concentrations are suggested starting points. Titration of the Vinculin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Recombinant human protein was used as the immunogen for the Vinculin antibody.

Storage

Aliquot the Vinculin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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