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Home >> Antibodies >> Vinculin Antibody

Vinculin Antibody (F54510)

  Catalog No Formulation Size Price (USD)  
Image F54510-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 439
Image
F54510-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 205
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Flow cytometry testing of fixed and permeabilized mouse NIH 3T3 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.
Flow cytometry testing of fixed and permeabilized mouse C2C12 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.
Western blot testing of 1) human skeletal muscle, 2) human A431, 3) human HeLa, 4) mouse kidney, 5) rat PC-12 and 6) human HepG2 cell lysate with Vinculin antibody. Predicted molecular weight ~124 kDa.
IHC testing of FFPE mouse skeletal muscle tissue with Vinculin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q64727
Applications Western Blot : 1:500-1:2000
Flow Cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This Vinculin antibody is available for research use only.
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Description

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).

Learn more about focal adhesion biology and vinculin research by visiting our Vinculin Antibody / Focal Adhesion Protein Antibody page.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Vinculin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Recombinant human protein was used as the immunogen for the Vinculin antibody.

Storage

Aliquot the Vinculin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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