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Home >> Antibodies >> Vimentin Antibody

Vimentin Antibody [clone V9] (R30090)

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Image R30090 0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS 100 ug 429
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Immunofluorescent staining of FFPE human intestinal cancer with Vimentin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
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Immunofluorescent staining of FFPE human intestinal cancer with Vimentin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
IHC staining of FFPE human invasive urothelial carcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human invasive urothelial carcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC-P: Vimentin antibody testing of human breast cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE rat brain tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) A375, 3) U-87 MG and 4) HepG2 cell lysate with Vimentin antibody. Predicted molecular weight ~54 kDa.
Availability 1-3 business days
Species Reactivity Human, Rabbit, Pig, Rat
Format Ascites
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name V9
Gene ID 7431
Localization Cytoplasmic, nuclear, cell membrane
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Limitations This Vimentin antibody is available for research use only.
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Description

The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. The Vimentin gene expresses in human lymphocytes and in Burkitts lymphoma cells and it is secreted by activated macrophages. The gene encoding human protein is located on the short arm of chromosome 10.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Pig eye lens vimentin was used as the immunogen for this Vimentin antibody.

Storage

After reconstitution, the Vimentin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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