Vimentin Antibody / Mechanotransduction Marker Antibody [clone VIM/3736] (V8161)

Vimentin Antibody / Mechanotransduction Marker Antibody. Immunohistochemistry analysis of FFPE human colon carcinoma tissue using Vimentin antibody shows strong HRP-DAB brown cytoplasmic staining in stromal and spindle-shaped mesenchymal cells within the tumor microenvironment, with minimal staining in most tumor epithelial cells. The rabbit monoclonal clone VIM/3736 highlights elongated, filamentous cytoplasmic structures aligned along cellular axes, consistent with force-bearing cytoskeletal organization. The distribution and morphology of Vimentin-positive cells support its role in cytoskeletal tension and mechanical signaling within tumor-associated stromal compartments.

IHC staining of FFPE human prostate carcinoma with Vimentin antibody (clone VIM/3736). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Vimentin Antibody / Mechanotransduction Marker Antibody. HuProt microarray specificity analysis using Vimentin antibody demonstrates selective binding to Vimentin (VIM) among more than 19,000 full-length human proteins. The rabbit monoclonal clone VIM/3736 shows the highest signal intensity for VIM with a strong Z score and high S score, indicating high target specificity relative to other proteins on the array. This specificity supports reliable detection of Vimentin in studies of cytoskeletal tension and force-responsive cellular structures, consistent with its role in mechanotransduction-related processes.

SDS-PAGE analysis of purified, BSA-free Vimentin antibody (clone VIM/3736) as confirmation of integrity and purity.

Western blot testing of human U-87 MG cell lysate with Vimentin antibody. Expected molecular weight: 53-58 kDa.