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Home >> Antibodies >> Recombinant Vimentin Antibody

Recombinant Vimentin Antibody [clone VIM/6430R] (V9284)

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  Catalog No Formulation Size Price (USD)  
Image V9284-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9284-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9284SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE dog kidney tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE cat kidney tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE horse small intestine tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE horse lung tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE cow brain tissue with recombinant Vimentin antibody (clone VIM/6430R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human U-87 MG cell lysate with recombinant Vimentin antibody. Predicted molecular weight ~53 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant Vimentin antibody (clone VIM/6430R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Dog, Cat, Cow, Horse
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name VIM/6430R
Purity Protein A/G affinity
UniProt P08670
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant Vimentin antibody is available for research use only.
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Description

Anti-Vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Application Notes

Optimal dilution of the recombinant Vimentin antibody should be determined by the researcher.

Immunogen

A portion of amino acids 2-466 from the human protein was used as the immunogen for the recombinant Vimentin antibody.

Storage

Aliquot the recombinant Vimentin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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