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Home >> Antibodies >> Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody

Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody (R31772)

  Catalog No Formulation Size Price (USD)  
Image R31772 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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Vimentin Antibody / Tumor Microenvironment Marker Antibody Human Breast Cancer IHC. Immunohistochemical staining of FFPE human breast cancer tissue using Vimentin Antibody / Tumor Microenvironment Marker Antibody. Vimentin expression is observed predominantly within stromal fibroblasts and other mesenchymal cells of the tumor microenvironment, consistent with its role in cytoskeletal remodeling, cell migration, and stromal support of tumor progression. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 minutes followed by cooling prior to immunostaining.
Vimentin Antibody / Tumor Microenvironment Marker Antibody Mouse Heart IHC. Immunohistochemical staining of FFPE mouse heart tissue using Vimentin Antibody / Tumor Microenvironment Marker Antibody. Vimentin expression is detected predominantly in vascular endothelial cells and interstitial stromal cells, consistent with its role as a marker of the cardiac stromal microenvironment and its involvement in cytoskeletal organization, tissue remodeling, and vascular integrity. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 minutes followed by cooling prior to immunostaining.
Vimentin Antibody / Tumor Microenvironment Marker Antibody Rat Heart IHC. Immunohistochemical staining of FFPE rat heart tissue using Vimentin Antibody / Tumor Microenvironment Marker Antibody. Vimentin expression is detected predominantly in vascular endothelial cells and surrounding stromal cells, consistent with its role as a marker of the cardiac microenvironment and its involvement in cytoskeletal organization, vascular homeostasis, and tissue remodeling. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 minutes followed by cooling prior to immunostaining.
Vimentin Antibody / Tumor Microenvironment Marker Antibody Human Placenta IHC. Immunohistochemical staining of FFPE human placental tissue using Vimentin Antibody / Tumor Microenvironment Marker Antibody. Vimentin expression is observed predominantly within stromal cells and vascular components of the placental microenvironment, consistent with its role in maintaining structural integrity, supporting mesenchymal cell function, and facilitating tissue remodeling during placental development. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 minutes followed by cooling prior to immunostaining.
Vimentin Antibody / Tumor Microenvironment Marker Antibody Human Tonsil IHC. Immunohistochemical staining of FFPE human tonsil tissue using Vimentin Antibody / Tumor Microenvironment Marker Antibody. Vimentin expression is observed predominantly within stromal cells, vascular endothelial cells, and supporting mesenchymal cells of the tonsillar microenvironment, consistent with its role in maintaining tissue architecture, immune cell support, and extracellular matrix remodeling. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 minutes followed by cooling prior to immunostaining.
Vimentin Antibody / Tumor Microenvironment Marker Antibody Human Lung Adenocarcinoma IHC. Immunohistochemical staining of FFPE human lung adenocarcinoma tissue using Vimentin Antibody / Tumor Microenvironment Marker Antibody. Vimentin expression is observed predominantly within stromal fibroblasts, vascular elements, and other mesenchymal cells of the tumor microenvironment, consistent with its role in supporting extracellular matrix remodeling, epithelial-to-mesenchymal transition, and tumor progression. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 minutes followed by cooling prior to immunostaining.
Vimentin Antibody / Tumor Microenvironment Marker Antibody Human Colorectal Adenocarcinoma IHC. Immunohistochemical staining of FFPE human colorectal adenocarcinoma tissue using Vimentin Antibody / Tumor Microenvironment Marker Antibody. Vimentin expression is observed predominantly within stromal fibroblasts and other mesenchymal cells surrounding the tumor glands, consistent with its role as a marker of the tumor microenvironment involved in extracellular matrix remodeling, stromal activation, and tumor progression. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 minutes followed by cooling prior to immunostaining.
Vimentin Antibody / Tumor Microenvironment Marker Antibody Human Invasive Urothelial Carcinoma IHC. Immunohistochemical staining of FFPE human invasive urothelial carcinoma tissue using Vimentin Antibody / Tumor Microenvironment Marker Antibody. Vimentin expression is observed predominantly within stromal fibroblasts, infiltrating mesenchymal cells, and vascular elements of the tumor microenvironment, consistent with its role in extracellular matrix remodeling, epithelial-to-mesenchymal transition, and the invasive behavior of urothelial carcinoma. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 minutes followed by cooling prior to immunostaining.
Vimentin Antibody / Tumor Microenvironment Marker Antibody Human Liver Cancer IHC. Immunohistochemical staining of FFPE human liver cancer tissue using Vimentin Antibody / Tumor Microenvironment Marker Antibody. Vimentin expression is observed predominantly within stromal fibroblasts, vascular endothelial cells, and other mesenchymal components of the tumor microenvironment, consistent with its role in extracellular matrix remodeling, angiogenesis, and support of tumor progression. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 minutes followed by cooling prior to immunostaining.
Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody. Immunofluorescence analysis of Vimentin / VIM in mouse brain tissue demonstrating green cytoplasmic and filamentous staining in stromal and supporting cell populations. The Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody used as a tumor microenvironment immunofluorescence marker highlights Vimentin-positive cells with elongated morphology and network-like distribution within the tissue. Nuclei are counterstained with DAPI (blue), supporting visualization of stromal architecture and microenvironment organization.
Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody. Immunofluorescence analysis of Vimentin / VIM in rat brain tissue demonstrating green cytoplasmic and filamentous staining in stromal and supporting cell populations, including cells lining vascular and parenchymal structures. The Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody. highlights Vimentin-positive cells with elongated morphology and network-like distribution within the tissue, consistent with microenvironment-associated cell populations. Nuclei are counterstained with DAPI (blue), supporting visualization of stromal architecture and tissue organization.
Vimentin Antibody / Tumor Microenvironment Marker Antibody Multi-Species WB. Western blot analysis of 1) human placenta, 2) human U-87 MG, 3) human U-2 OS, 4) human PC-3, 5) human HeLa, 6) human ThP-1, 7) human A549, 8) human MCF7, 9) rat ovary, 10) rat lung, 11) mouse lung, and 12) mouse NIH 3T3 lysates using Vimentin Antibody / Tumor Microenvironment Marker Antibody. A prominent immunoreactive band is detected at approximately 54 kDa across all samples, consistent with the predicted molecular weight of vimentin, supporting specific detection of this type III intermediate filament protein in human, rat, and mouse tissues and cell lines.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Gene ID 7431
Localization Cytoplasmic
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Limitations This Vimentin antibody is available for research use only.
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Description

Vimentin (VIM) is a type III intermediate filament protein that is highly expressed in mesenchymal cells and is a defining marker of the tumor microenvironment. Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker is specifically positioned for immunofluorescence-based visualization of stromal and tumor-associated cell populations, enabling high-resolution analysis of tumor microenvironment architecture at the single-cell level. This aligns strongly with search intent such as Vimentin immunofluorescence antibody, VIM antibody for immunofluorescence, and tumor microenvironment immunofluorescence marker. Vimentin is also referred to as Vimentin antibody, VIM antibody, stromal marker antibody, and fibroblast marker antibody in the literature, strengthening alignment across gene, protein, and pathology-focused terminology.

In immunofluorescence imaging, Vimentin is prominently expressed in tumor-associated fibroblasts, endothelial cells, and other stromal components that surround and support tumor growth. The Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker enables clear visualization of these Vimentin-positive stromal networks, which often appear as interconnected cytoplasmic filament structures distributed throughout the tumor microenvironment. This spatial resolution is critical for distinguishing tumor cells from surrounding stromal populations and for mapping cellular organization within complex tissue systems.

Vimentin plays a central role in the biology of cancer-associated fibroblasts and other stromal cells that regulate tumor progression, invasion, and therapeutic response. In fluorescence-based workflows, the Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker supports detailed analysis of stromal activation, tumor-stroma interactions, and tissue remodeling processes. It is particularly valuable in co-localization studies, where Vimentin expression is evaluated alongside epithelial, immune, or vascular markers to define distinct cellular compartments within the tumor microenvironment.

Functionally, Vimentin interacts with actin filaments and microtubules to regulate cytoskeletal organization, cell migration, and mechanical signaling within stromal cells. Its expression contributes to extracellular matrix remodeling and structural support of tumor tissues. The Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker differentiator is central to this antibody, positioning it specifically for imaging-based studies of stromal biology and tumor microenvironment organization rather than cytoskeletal architecture alone or EMT-focused transitions. A rabbit polyclonal Vimentin Antibody provides strong fluorescent signal and enables high-resolution visualization of Vimentin / VIM-positive stromal networks in complex tumor tissues and microenvironment-focused research models.

Explore our Protein Microarray Validated Vimentin Antibody page to learn more about vimentin biology, mesenchymal cell markers, and additional validated products.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human Vimentin (DTHSKRTLLIKTVETRDGQVINETSQHHDDLE) was used as the immunogen for this Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the Vimentin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

VIM antibody, Vimentin immunofluorescence antibody, Stromal marker antibody, Fibroblast marker antibody, Tumor microenvironment marker antibody

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