Vimentin Antibody-Tumor Microenvironment IF Marker Ab-R31772

	Catalog No	Formulation	Size	Price (USD)
	R31772	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

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IHC-P: Vimentin antibody testing of human breast cancer tissue. Required HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC-P: Vimentin antibody testing of mouse heart. Required HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC-P: Vimentin antibody testing of rat heart. Required HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human placental tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung adenocarcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colorectal adenocarcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human invasive urothelial carcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody. Immunofluorescence analysis of Vimentin / VIM in mouse brain tissue demonstrating green cytoplasmic and filamentous staining in stromal and supporting cell populations. The Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody used as a tumor microenvironment immunofluorescence marker highlights Vimentin-positive cells with elongated morphology and network-like distribution within the tissue. Nuclei are counterstained with DAPI (blue), supporting visualization of stromal architecture and microenvironment organization.

Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody. Immunofluorescence analysis of Vimentin / VIM in rat brain tissue demonstrating green cytoplasmic and filamentous staining in stromal and supporting cell populations, including cells lining vascular and parenchymal structures. The Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody. highlights Vimentin-positive cells with elongated morphology and network-like distribution within the tissue, consistent with microenvironment-associated cell populations. Nuclei are counterstained with DAPI (blue), supporting visualization of stromal architecture and tissue organization.

Western blot testing of 1) human placenta, 2) human U-87 MG, 3) human U-2 OS, 4) human PC-3, 5) human HeLa, 6) human ThP-1, 7) human A549, 8) human MCF7, 9) rat ovary, 10) rat lung, 11) mouse lung and 12) mouse NIH 3T3 cell lysate with Vimentin antibody. Predicted molecular weight ~54 kDa.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity
Buffer	Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Gene ID	7431
Localization	Cytoplasmic
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml
Limitations	This Vimentin antibody is available for research use only.
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Description

Vimentin (VIM) is a type III intermediate filament protein that is highly expressed in mesenchymal cells and is a defining marker of the tumor microenvironment. Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker is specifically positioned for immunofluorescence-based visualization of stromal and tumor-associated cell populations, enabling high-resolution analysis of tumor microenvironment architecture at the single-cell level. This aligns strongly with search intent such as Vimentin immunofluorescence antibody, VIM antibody for immunofluorescence, and tumor microenvironment immunofluorescence marker. Vimentin is also referred to as Vimentin antibody, VIM antibody, stromal marker antibody, and fibroblast marker antibody in the literature, strengthening alignment across gene, protein, and pathology-focused terminology.

In immunofluorescence imaging, Vimentin is prominently expressed in tumor-associated fibroblasts, endothelial cells, and other stromal components that surround and support tumor growth. The Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker enables clear visualization of these Vimentin-positive stromal networks, which often appear as interconnected cytoplasmic filament structures distributed throughout the tumor microenvironment. This spatial resolution is critical for distinguishing tumor cells from surrounding stromal populations and for mapping cellular organization within complex tissue systems.

Vimentin plays a central role in the biology of cancer-associated fibroblasts and other stromal cells that regulate tumor progression, invasion, and therapeutic response. In fluorescence-based workflows, the Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker supports detailed analysis of stromal activation, tumor-stroma interactions, and tissue remodeling processes. It is particularly valuable in co-localization studies, where Vimentin expression is evaluated alongside epithelial, immune, or vascular markers to define distinct cellular compartments within the tumor microenvironment.

Functionally, Vimentin interacts with actin filaments and microtubules to regulate cytoskeletal organization, cell migration, and mechanical signaling within stromal cells. Its expression contributes to extracellular matrix remodeling and structural support of tumor tissues. The Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker differentiator is central to this antibody, positioning it specifically for imaging-based studies of stromal biology and tumor microenvironment organization rather than cytoskeletal architecture alone or EMT-focused transitions. A rabbit polyclonal Vimentin Antibody provides strong fluorescent signal and enables high-resolution visualization of Vimentin / VIM-positive stromal networks in complex tumor tissues and microenvironment-focused research models.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human Vimentin (DTHSKRTLLIKTVETRDGQVINETSQHHDDLE) was used as the immunogen for this Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the Vimentin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

VIM antibody, Vimentin immunofluorescence antibody, Stromal marker antibody, Fibroblast marker antibody, Tumor microenvironment marker antibody

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Vimentin Antibody / Tumor Microenvironment Immunofluorescence Marker Antibody (R31772)

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