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- Tel: 858.663.9055
- Email: info@nsjbio.com
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Vimentin, also known as HEL113 or CTRCT30, is encoded by the VIM gene and is a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
The stated application concentrations are suggested starting points. Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An amino acid sequence from the C-terminus of human Vimentin (DTHSKRTLLIKTVETRDGQVINETSQHHDDLE) was used as the immunogen for this Vimentin antibody (100% homologous in human, mouse and rat).
After reconstitution, the Vimentin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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