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Home >> Antibodies >> Vimentin Antibody

Vimentin Antibody (F48161)

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  Catalog No Formulation Size Price (USD)  
Image F48161-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48161-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Fluorescent confocal image of SY5Y cells stained with Vimentin antibody at 1:200. The immunosignal is localized to the cytoskeleton.
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Fluorescent confocal image of SY5Y cells stained with Vimentin antibody at 1:200. The immunosignal is localized to the cytoskeleton.
Western blot analysis of Vimentinin antibody and HeLa lysate
Western blot analysis of Vimentin antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (2).
IHC analysis of FFPE human breast carcinoma tissue stained with Vimentin antibody
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Bovine, Hamster, Primate, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P08670
Applications Immunofluorescence : 1:200
Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Limitations This Vimentin antibody is available for research use only.
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Description

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Application Notes

Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 63-90 from the human protein was used as the immunogen for this Vimentin antibody.

Storage

Aliquot the Vimentin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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