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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Vimentin Antibody for WB / VIM Western Blot Antibody [clone 4F8-A5-H5] (F54035)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
F54035-0.1ML | In PBS with 50% glycerol and 0.03% ProClin 300 | 0.1 ml | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2a |
| Clone Name | 4F8-A5-H5 |
| Purity | Protein G affinity |
| UniProt | P08670 |
| Applications | Western Blot : 1:1000 ICC/IF : 1:800 Immunoprecipitation : suitable |
| Limitations | This Vimentin antibody is available for research use only. |
|
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Vimentin (VIM) is a type III intermediate filament protein that is highly expressed in mesenchymal cells and serves as a key cytoskeletal component regulating cellular structure and stability. Vimentin Antibody for WB is specifically designed for western blot analysis, enabling robust detection of Vimentin protein bands in cell and tissue lysates under denaturing conditions. This aligns strongly with search intent such as Vimentin western blot antibody, VIM WB antibody, and Vimentin antibody for SDS-PAGE analysis. Vimentin is also referred to as Vimentin antibody, VIM antibody, and intermediate filament marker antibody in the literature, supporting strong alignment between gene, protein, and assay-specific terminology.
In western blot workflows, Vimentin Antibody for WB enables clear identification of Vimentin bands across a wide range of biological samples, including fibroblasts, endothelial cells, leukocytes, and mesenchymal-derived tumor cell lysates. The antibody supports detection of Vimentin under reducing and denaturing conditions, making it suitable for SDS-PAGE separation followed by immunoblotting. This application is essential for confirming protein expression, comparing sample-to-sample variation, and validating mesenchymal phenotype at the protein level.
Vimentin is subject to post-translational modifications such as phosphorylation, which can influence its electrophoretic mobility and result in band pattern variation on western blot. Vimentin Antibody for WB is therefore useful for analyzing changes in protein state, cytoskeletal remodeling, and signaling-dependent modifications across experimental conditions. It is commonly used in studies of epithelial-mesenchymal transition, tumor progression, and cytoskeletal dynamics where protein-level validation is required.
Functionally, Vimentin interacts with actin filaments and microtubules to coordinate cytoskeletal organization, cell migration, and intracellular transport. The Vimentin Antibody for WB differentiator is central to this antibody, positioning it specifically for protein detection, band analysis, and comparative expression studies rather than imaging-based applications. A mouse monoclonal Vimentin Antibody for WB provides strong and consistent signal detection, supporting reliable visualization of Vimentin / VIM bands in western blot assays across diverse sample types and experimental conditions.
The stated application concentrations are suggested starting points. Titration of the Vimentin Antibody for WB / VIM Western Blot Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A human recombinant partial protein was used as the immunogen for this Vimentin Antibody for WB / VIM Western Blot Antibody.
Store the Vimentin antibody at -20oC.
VIM antibody, Vimentin western blot antibody, Mesenchymal marker antibody, Intermediate filament protein antibody, Cytoskeletal marker antibody
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