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Home >> Antibodies >> VIM Antibody / Vimentin

VIM Antibody / Vimentin [clone MSVA-458R] (V6129)

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  Catalog No Formulation Size Price (USD)  
Image V6129-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 599.50
Image
V6129-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
Microvalidated Recrabbitmono
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Vimentin Rabbit Recombinant Monoclonal Antibody (MSVA-458R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-458R
UniProt P08670
Localization Cell membrane, Cytoplasm, Cytoskeleton, Nucleus matrix
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This VIM/Vimentin antibody is available for research use only.
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Application Notes

1. Optimal dilution of the VIM/Vimentin antibody should be determined by the researcher.

2. This VIM/Vimentin antibody is recombinantly produced by expression in human HEK293 cells.

Immunogen

A recombinant fragment (around amino acids 2-466) of human Vimentin protein (exact sequence is proprietary) was used as the immuongen for the VIM/Vimentin antibody.

Storage

VIM/Vimentin antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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