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Home >> Antibodies >> VIM Antibody

VIM Antibody (F48163)

  Catalog No Formulation Size Price (USD)  
F48163-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329

F48163-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Western blot analysis of VIM antibody and A2058, A375 lysate.
Western blot analysis of VIM antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (2).
IHC analysis of FFPE human breast carcinoma tissue stained with VIM antibody
VIM antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue.
VIM antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC secondary Ab was used.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat, Bovine, Pig, Hamster
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P08670
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This VIM antibody is available for research use only.
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Description

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the VIM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 152-181 from the human protein was used as the immunogen for this VIM antibody.

Storage

For long term storage, aliquot the VIM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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