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Home >> Antibodies >> Villin-like protein Antibody / VILL

Villin-like protein Antibody / VILL (F54634)

  Catalog No Formulation Size Price (USD)  
Image F54634-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54634-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human K562 cell lysate with Villin-like protein antibody. Predicted molecular weight ~96 kDa.
IHC testing of FFPE human skeletal muscle tissue with Villin-like protein antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human WiDr cells with Villin-like protein antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human K562 cells with Villin-like protein antibody; Blue=isotype control, Green= Villin-like protein antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt O15195
Localization Cytoplasmic
Applications Flow cytometry : 1:25 (1x10e6 cells)
Immunofluorescence : 1:25
Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Limitations This Villin-like protein antibody is available for research use only.
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Description

The protein encoded by this gene belongs to the villin/gelsolin family. It contains 6 gelsolin-like repeats and a headpiece domain. It may play a role in actin-bundling. [RefSeq].

Application Notes

The stated application concentrations are suggested starting points. Titration of the Villin-like protein antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 385-412 from the human protein was used as the immunogen for the Villin-like protein antibody.

Storage

Aliquot the Villin-like protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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