Villin Antibody for IF / VIL1 Immunofluorescence Antibody (R31923)

Western blot testing of 1) rat intestine, 2) mouse kidney, 3) human RH35, 4) HepG2 and 5) MCF7 lysate with Villin antibody. Expected/observed molecular weight ~93 kDa.

IHC testing of FFPE human intestinal cancer with Villin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE mouse intestine with Villin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE rat intestine with Villin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

Villin Antibody for IF. Immunofluorescence analysis of Villin-1 (VIL1) in FFPE human ileum using a VIL1 Immunofluorescence Antibody (rabbit polyclonal). Villin Antibody for IF produces strong apical staining of epithelial cells lining the intestinal villi (green), clearly highlighting brush border localization and epithelial polarity, while nuclei are counterstained with DAPI (blue). The distinct membrane-associated signal along the luminal surface is consistent with the known distribution of Villin in microvilli-rich absorptive epithelium, supporting its use for high-resolution immunofluorescence imaging of epithelial structure and apical cytoskeletal organization.

Villin Antibody for IF. Immunofluorescence analysis of Villin-1 (VIL1) in FFPE human colon using a VIL1 Immunofluorescence Antibody (rabbit polyclonal). Villin Antibody for IF shows strong, continuous apical membrane staining of colonic epithelial cells (green), clearly outlining the luminal brush border and emphasizing epithelial polarity, while nuclei are counterstained with DAPI (blue). The sharp, linear fluorescence along the glandular lumen highlights microvillus-rich absorptive surfaces, supporting use of this antibody for high-resolution immunofluorescence imaging of epithelial architecture and brush border cytoskeletal organization.

Villin Antibody for IF. Immunofluorescence analysis of Villin-1 (VIL1) in FFPE rectal cancer tissue using a VIL1 Immunofluorescence Antibody (rabbit polyclonal). Villin Antibody for IF demonstrates prominent apical membrane staining of tumor epithelial cells (green), outlining glandular structures and highlighting retained brush border-like polarity within malignant tissue, while nuclei are counterstained with DAPI (blue). The localized luminal fluorescence supports detection of epithelial differentiation patterns and is consistent with Villin expression in intestinal-type adenocarcinoma, making this antibody well suited for immunofluorescence imaging of tumor architecture and epithelial organization.

Villin Antibody for IF. Immunofluorescence analysis of Villin-1 (VIL1) in FFPE mouse intestine using a VIL1 Immunofluorescence Antibody (rabbit polyclonal). Villin Antibody for IF shows intense, continuous apical membrane staining of intestinal epithelial cells (green), sharply delineating the brush border along villus structures and clearly highlighting epithelial polarity, while nuclei are counterstained with DAPI (blue). The strong luminal fluorescence pattern is consistent with high Villin expression in microvilli-rich absorptive epithelium, supporting use of this antibody for high-resolution immunofluorescence imaging of epithelial architecture and apical cytoskeletal organization.

Villin Antibody for IF. Immunofluorescence analysis of Villin-1 (VIL1) in FFPE rat intestine using a VIL1 Immunofluorescence Antibody (rabbit polyclonal). Villin Antibody for IF demonstrates strong, continuous apical membrane staining of intestinal epithelial cells (green), clearly outlining villus structures and emphasizing brush border localization and epithelial polarity, while nuclei are counterstained with DAPI (blue). The sharp luminal fluorescence pattern is consistent with high Villin expression in microvilli-rich absorptive epithelium, supporting use of this antibody for high-resolution immunofluorescence imaging of epithelial architecture and apical cytoskeletal organization.