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Home >> Antibodies >> Villin Antibody / VIL1

Villin Antibody / VIL1 [clone VIL1/1325] (V3369)

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  Catalog No Formulation Size Price (USD)  
Image V3369-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3369-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3369SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human colon with Villin antibody (clone VIL1/1325). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human colon with Villin antibody (clone VIL1/1325). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human rectum tissue with Villin antibody (clone VIL1/1325). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human 1) A549, 2) HepG2 and 3) HCT-116 cell lysate with Villin antibody (clone VIL1/1325). Expected molecular weight ~93 kDa.
SDS-PAGE Analysis of Purified, BSA-Free Villin Antibody (clone VIL1/1325). Confirmation of Integrity and Purity of the Antibody.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name VIL1/1325
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt P09327
Localization Cytoplasmic and cell surface
Applications Western blot : 1-2ug/ml for 60 min at RT
Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Limitations This Villin antibody is available for research use only.
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Description

Villin (VIL1) is an epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the Villin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.

Storage

Store the Villin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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