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Home >> Antibodies >> VIL1 Antibody / Villin 1

VIL1 Antibody / Villin 1 [clone MSVA-459R] (V6128)

  Catalog No Formulation Size Price (USD)  
Image V6128-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 599.50
Image
V6128-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
Microvalidated Recrabbitmono
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Villin-1 Rabbit Recombinant Monoclonal Antibody (MSVA-459R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-459R
UniProt P09327
Localization Cell projection, Cytoplasm, Cytoskeleton, Filopodium, Filopodium tip, Lamellipodium, Microvillus, Ruffle
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This VIL1/Villin 1 antibody is available for research use only.
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Application Notes

1. Optimal dilution of the VIL1/Villin 1 antibody should be determined by the researcher.

2. This VIL1/Villin 1 antibody is recombinantly produced by expression in human HEK293 cells.

Immunogen

A recombinant fragment (around amino acids 600-700) of human Villin protein (exact sequence is proprietary) was used as the immuongen for the VIL1/Villin 1 antibody.

Storage

VIL1/Villin 1 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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