VIL1 Antibody for WB / Villin Western Blot Antibody (F54302)

VIL1 Antibody for WB. Western blot analysis of Villin-1 (VIL1) protein expression in multiple epithelial-derived lysates using a rabbit polyclonal antibody. Lane 1: human HT-29 lysate, Lane 2: human HepG2 lysate, Lane 3: human COLO205 lysate, Lane 4: mouse colon lysate. A band is detected at approximately 93 kDa using the Villin Western Blot Antibody, consistent with the predicted molecular weight of Villin-1 / VIL1. This banding pattern aligns with the known enrichment of Villin in epithelial cells and supports its role as a brush border cytoskeletal marker commonly detected in western blot analysis of intestinal and epithelial-derived samples.

VIL1 Antibody for WB. Western blot analysis of Villin-1 (VIL1) protein expression in epithelial and epithelial-derived lysates using a rabbit polyclonal antibody. Lane 1: human HepG2 lysate, Lane 2: human HT-29 lysate, Lane 3: mouse kidney lysate. A band is detected at approximately 93 kDa with the Villin Western Blot Antibody, consistent with the predicted molecular weight of Villin-1 / VIL1. The strong signal in epithelial-derived cell lines and detectable expression in kidney lysate align with the known distribution of Villin in polarized epithelial tissues, supporting its use as a reliable western blot marker of brush border-associated cytoskeletal structure and epithelial differentiation.

Western blot testing of human 1) Caco-2 and 2) HT-29 cell lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.

VIL1 Antibody for WB. Western blot analysis of Villin-1 (VIL1) protein expression in epithelial-derived lysates using a rabbit polyclonal antibody. Lane 1: human HT-29 lysate, Lane 2: human SW480 lysate, Lane 3: mouse colon lysate. A band is detected at approximately 93 kDa, consistent with the predicted molecular weight of Villin-1 / VIL1. Additional lower molecular weight bands are observed in some lanes, which may reflect proteolytic processing or partial degradation products commonly seen in cytoskeletal proteins under certain lysis conditions. The strong signal in colon-derived samples aligns with the known enrichment of Villin in intestinal epithelium, supporting its use as a robust western blot marker for epithelial brush border structure and differentiation.

IHC testing of FFPE human kidney tissue with VIL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry testing of fixed and permeabilized human HeLa cells with VIL1 antibody; Blue=isotype control, Green= VIL1 antibody.