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Home >> Antibodies >> VIL1 Antibody / Villin 1

VIL1 Antibody / Villin 1 (F54302)

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  Catalog No Formulation Size Price (USD)  
Image F54302-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54302-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of 1) human HT-29, 2) human HepG2, 3) human COLO205 and 4) mouse colon lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.
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Western blot testing of 1) human HT-29, 2) human HepG2, 3) human COLO205 and 4) mouse colon lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.
Western blot testing of 1) human HepG2, 2) human HT-29 and 3) mouse kidney lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.
Western blot testing of human 1) Caco-2 and 2) HT-29 cell lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.
Western blot testing of 1) human HT-29, 2) human SW480 and 3) mouse colon lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.
IHC testing of FFPE human kidney tissue with VIL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human HeLa cells with VIL1 antibody; Blue=isotype control, Green= VIL1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P09327
Localization Cytoplasmic and cell surface
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Limitations This VIL1 antibody is available for research use only.
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Description

Villin-1 is a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments.

Application Notes

The stated application concentrations are suggested starting points. Titration of the VIL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 180-207 from the human protein were used as the immunogen for the VIL1 antibody.

Storage

Aliquot the VIL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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