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Home >> Antibodies >> VHL Antibody

VHL Antibody (F49681)

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  Catalog No Formulation Size Price (USD)  
Image F49681-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
Image
F49681-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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VHL antibody IHC analysis in formalin fixed and paraffin embedded mouse brain tissue.
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VHL antibody IHC analysis in formalin fixed and paraffin embedded mouse brain tissue.
Confocal immunofluorescent analysis of VHL antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
Western blot analysis of VHL antibody and HepG2 lysate.
VHL antibody flow cytometric analysis of HepG2 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
  • VHL antibody IHC analysis in formalin fixed and paraffin embedded mouse brain tissue.
  • Confocal immunofluorescent analysis of VHL antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
  • Western blot analysis of VHL antibody and HepG2 lysate.
  • VHL antibody flow cytometric analysis of HepG2 cells (green) compared to a <a href=negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis." title="VHL antibody flow cytometric analysis of HepG2 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis." onClick="showhideiamges('image4','imagelargedata4',4,4)">
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P40337
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This VHL antibody is available for research use only.
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Description

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of VHL gene is the basis of familial inheritance of VHL syndrome. The protein is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein.

Application Notes

Titration of the VHL antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 43-71 from the human protein was used as the immunogen for this VHL antibody.

Storage

Aliquot the VHL antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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