VHL Antibody / Tumor Suppressor Protein Antibody (F49681)

VHL Antibody Mouse Brain IHC. Immunohistochemistry staining of FFPE mouse brain tissue using VHL antibody demonstrates widespread cytoplasmic staining throughout neuronal populations, consistent with expression of Von Hippel-Lindau protein (VHL). VHL is a tumor suppressor protein that regulates hypoxia-inducible factor (HIF) signaling and plays a central role in cellular oxygen sensing, metabolic adaptation, and maintenance of cellular homeostasis. The observed staining pattern is consistent with the established role of VHL in regulating oxygen-responsive pathways within metabolically active neural tissues. These findings support the utility of VHL antibody for studies of hypoxia signaling, HIF regulation, and tumor suppressor-associated cellular pathways in the nervous system.

VHL Antibody HepG2 Cell IF. Immunofluorescent staining of HepG2 cells using VHL antibody demonstrates punctate cytoplasmic and perinuclear localization of Von Hippel-Lindau protein (VHL), shown in green. VHL is a tumor suppressor protein that regulates hypoxia-inducible factor (HIF) signaling and serves as a critical component of cellular oxygen-sensing pathways. The observed intracellular distribution is consistent with the role of VHL in protein degradation complexes and regulation of hypoxia-responsive signaling networks. Actin filaments are visualized with Alexa Fluor 555 Phalloidin (red), while DAPI (blue) identifies cell nuclei. The staining pattern supports the utility of VHL antibody for studies of tumor suppression, hypoxia signaling, and oxygen homeostasis pathways.

VHL Antibody HepG2 WB. Western blot analysis of HepG2 cell lysate using VHL antibody demonstrates detection of an immunoreactive band at approximately 18-20 kDa, consistent with the expected molecular weight of Von Hippel-Lindau protein (VHL). VHL is a tumor suppressor protein that functions as a key regulator of hypoxia-inducible factor (HIF) signaling and cellular oxygen-sensing pathways. Through its role in an E3 ubiquitin ligase complex, VHL controls degradation of HIF proteins and helps maintain normal cellular responses to oxygen availability. The observed band supports the utility of this antibody for studies of tumor suppression, hypoxia signaling, oxygen homeostasis, and HIF-regulated cellular pathways. Predicted molecular weight: ~18-24 kDa.

VHL Antibody HepG2 Flow Cytometry. Flow cytometric analysis of HepG2 cells using VHL antibody demonstrates a clear rightward shift of the green histogram (VHL antibody) relative to the blue histogram (negative control), consistent with expression of Von Hippel-Lindau protein (VHL). VHL is a tumor suppressor protein that regulates hypoxia-inducible factor (HIF) signaling and serves as a central component of cellular oxygen-sensing pathways. The observed staining pattern supports detection of endogenous VHL expression and demonstrates the utility of this antibody for studies of tumor suppression, hypoxia signaling, oxygen homeostasis, and HIF-regulated cellular responses. A FITC-conjugated goat anti-rabbit secondary antibody was used for detection.