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Home >> Antibodies >> Versican Antibody

Versican Antibody (R31959)

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  Catalog No Formulation Size Price (USD)  
Image R31959 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
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Western blot testing of 1) rat brain and 2) human HEPA lysate with Versican antibody. Predicted molecular weight: ~373/265/182/74/370 kDa (isoforms V0, V1, V2, V3 and Vint)
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Western blot testing of 1) rat brain and 2) human HEPA lysate with Versican antibody. Predicted molecular weight: ~373/265/182/74/370 kDa (isoforms V0, V1, V2, V3 and Vint)
IHC testing of FFPE human glioma tissue with Versican antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P13611
Localization Intracellular, secreted
Applications Western blot : 0.1-0.5ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Limitations This Versican antibody is available for research use only.
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Description

Versican (VCAN), also known as CSPG2, is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Versican is a large chondroitin sulfate proteoglycan. The Versican gene is mapped on 5q14.2-q14.3. The human versican gene contains 15 exons spanning more than 90 kb. The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican staining was noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, which is upregulated in many human tumors including lung cancer, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14. By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, versican strongly enhances LLC metastatic growth.

Application Notes

Optimal dilution of the Versican antibody should be determined by the researcher.

Immunogen

Amino acids KWSKIEVDKNGKDLKETTVLVAQNGNIK of human Versican were used as the immunogen for the Versican antibody.

Storage

After reconstitution, the Versican antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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