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Home >> Antibodies >> VEGFR2 Antibody / KDR Polyclonal Antibody

VEGFR2 Antibody / KDR Polyclonal Antibody (R32871)

  Catalog No Formulation Size Price (USD)  
Image R32871 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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VEGFR2 Antibody in Human Intestinal Cancer IHC. Immunohistochemistry staining of FFPE human intestinal cancer tissue with VEGFR2 Antibody at 1 ug/ml. HIER was performed by steaming sections in pH 6 citrate buffer for 20 min followed by cooling before testing. Brown immunoreactivity is distributed through tumor-associated cellular and vascular structures, with nuclei counterstained blue. The staining pattern demonstrates detection of KDR protein in malignant intestinal tissue with the KDR Polyclonal Antibody.
VEGFR2 Antibody in Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer tissue with VEGFR2 Antibody at 1 ug/ml. HIER was performed by steaming sections in pH 6 citrate buffer for 20 min followed by cooling before testing. Strong brown immunoreactivity is particularly prominent along an elongated vascular structure, with additional staining present in surrounding tumor tissue. This vascular-associated pattern supports detection of VEGFR2 in human lung cancer with the KDR Polyclonal Antibody.
VEGFR2 Antibody in Human Breast Cancer IHC. Immunohistochemistry staining of FFPE human breast cancer tissue with VEGFR2 Antibody at 1 ug/ml. HIER was performed by steaming sections in pH 6 citrate buffer for 20 min followed by cooling before testing. Brown immunoreactivity is visible in clusters of malignant epithelial cells within the surrounding stromal tissue, with variation in staining intensity across the field. The cellular staining demonstrates detection of KDR with the KDR Polyclonal Antibody.
VEGFR2 Antibody in Human Placenta IHC. Immunohistochemistry staining of FFPE human placenta tissue with VEGFR2 Antibody at 1 ug/ml. HIER was performed by steaming sections in pH 6 citrate buffer for 20 min followed by cooling before testing. Distinct brown staining outlines multiple vascular structures, with particularly strong labeling of vessel-associated cells against comparatively weak surrounding tissue. This vascular staining pattern provides biologically relevant tissue validation for the KDR Polyclonal Antibody.
VEGFR2 Antibody in MCF7 Cells WB. Western blot testing of human MCF7 cell lysate with VEGFR2 Antibody at 0.5 ug/ml. The blot shows prominent immunoreactive bands at approximately 180 kDa and 100 kDa. VEGFR2 is expected to occur in differently processed and glycosylated forms, with the product page listing approximately 152 kDa for immature receptor, 180-200 kDa for intermediate forms and 220-230 kDa for mature receptor. The upper band is therefore compatible with a processed VEGFR2 species, while the lower band may represent an additional receptor form or nonspecific reactivity. This experiment provides western blot validation for the KDR Polyclonal Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt P35968
Localization Cytoplasmic and cell surface
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This VEGFR2 Antibody / KDR Polyclonal Antibody is available for research use only.
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Description

VEGFR2 Antibody / KDR Polyclonal Antibody recognizes vascular endothelial growth factor receptor 2, a receptor tyrosine kinase encoded by the KDR gene. VEGFR2 is a major signaling receptor for vascular endothelial growth factors and plays a central role in angiogenesis, vascular development and endothelial cell biology. The receptor is also known as KDR and CD309, while Flk1 is widely used for the corresponding mouse receptor.

VEGFR2 is a single-pass transmembrane protein containing an extracellular ligand-binding region, a transmembrane segment and an intracellular tyrosine kinase domain. Binding of VEGF promotes receptor dimerization and tyrosine phosphorylation, creating docking sites for signaling proteins that transmit VEGF-dependent signals into the cell. VEGF-A is the principal ligand associated with VEGFR2 activation, although additional VEGF family members can engage the receptor.

Signaling through KDR regulates endothelial cell proliferation, migration, survival and vascular permeability. Major downstream pathways include the MAPK/ERK, PI3K/AKT and phospholipase C gamma signaling cascades. Together, these pathways allow endothelial cells to respond to pro-angiogenic signals and contribute to the formation, remodeling and maintenance of blood vessels. VEGFR2 therefore occupies a central position in the molecular regulation of physiological and pathological angiogenesis.

VEGFR2 is extensively studied in cancer because tumor growth and metastatic progression can depend on development of an adequate blood supply. Increased VEGF signaling can promote tumor-associated angiogenesis, making the VEGF-VEGFR2 axis an important area of oncology and therapeutic research. KDR signaling is also relevant to developmental vascular biology, wound repair, inflammation and disorders involving abnormal vascular growth or permeability.

NSJ Bioreagents offers this VEGFR2 Antibody for research into VEGF signaling, receptor tyrosine kinase biology and angiogenesis. The KDR Polyclonal Antibody terminology provides the corresponding gene-based designation and distinguishes VEGFR2 from other members of the VEGF receptor family.

A VEGFR2 Antibody provides a polyclonal reagent for investigating vascular endothelial growth factor receptor 2 expression and signaling, while a KDR Polyclonal Antibody supports studies of angiogenesis, endothelial biology and VEGF-dependent pathways.

Explore additional antibodies involved in receptor signaling and intracellular regulatory pathways on our Signal Transduction Antibodies page.

Application Notes

Optimal dilution of the VEGFR2 Antibody / KDR Polyclonal Antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids A20-L242 was used as the immunogen for the VEGFR2 Antibody.

Storage

After reconstitution, the VEGFR2 Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

KDR antibody, VEGF Receptor 2 antibody, Vascular Endothelial Growth Factor Receptor 2 antibody, CD309 antibody, Kinase Insert Domain Receptor antibody

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