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Home >> Antibodies >> VEGFA Antibody

VEGFA Antibody (RQ4390)

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  Catalog No Formulation Size Price (USD)  
Image RQ4390 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) mouse thymus, 2) mouse HEPA1-6 and 3) rat C6 cell lysate with VEGFA antibody at 0.5ug/ml. Expected molecular weight 19~22 kDa (monomer) and 38~44 kDa (dimer). Banding may appear larger than predicted due to glycosylation.
Western blot testing of 1) mouse thymus, 2) mouse HEPA1-6 and 3) rat C6 cell lysate with VEGFA antibody at 0.5ug/ml. Expected molecular weight 19~22 kDa (monomer) and 38~44 kDa (dimer). Banding may appear larger than predicted due to glycosylation.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q00731
Applications Western blot : 0.5-1ug/ml
Direct ELISA : 0.1-0.5ug/ml (recombinant mouse protein) (BSA-free format available)
Limitations This VEGFA antibody is available for research use only.
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Description

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. [UniProt]

Application Notes

Optimal dilution of the VEGFA antibody should be determined by the researcher.

Immunogen

A mouse partial recombinant protein corresponding to amino acids A27-N190 was used as the immunogen for the VEGFA antibody.

Storage

After reconstitution, the VEGFA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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