VEGF Receptor 2 Antibody / VEGFR2 Polyclonal Antibody (RQ4055)

VEGF Receptor 2 Antibody in Rat Kidney IHC. Immunohistochemistry staining of FFPE rat kidney tissue with VEGF Receptor 2 Antibody at 1 ug/ml. HIER was performed by steaming tissue sections in pH 6 citrate buffer for 20 min followed by cooling before testing. Distinct brown staining is concentrated in vascular and glomerular-associated structures, with additional labeling visible in portions of the surrounding renal tissue. The localized staining pattern supports tissue detection of VEGFR2 with the VEGFR2 Polyclonal Antibody.

VEGF Receptor 2 Antibody in Mouse Kidney IHC. Immunohistochemistry staining of FFPE mouse kidney tissue with VEGF Receptor 2 Antibody at 1 ug/ml. HIER was performed by steaming tissue sections in pH 6 citrate buffer for 20 min followed by cooling before testing. Brown immunoreactivity is visible throughout renal tubular structures and within glomerular regions, producing a clearly defined staining pattern against the counterstained tissue. This experiment demonstrates mouse tissue recognition with the VEGFR2 Polyclonal Antibody.

VEGF Receptor 2 Antibody with Recombinant Mouse VEGFR2 WB. Western blot testing of recombinant mouse VEGFR2 protein with VEGF Receptor 2 Antibody at 0.5 ug/ml. A strong, discrete band is detected at approximately 180 kDa with minimal additional reactivity. The observed molecular weight is compatible with a glycosylated form of VEGFR2 and provides direct target validation using recombinant receptor protein. This clean target-associated signal supports western blot performance of the VEGFR2 Polyclonal Antibody.

VEGF Receptor 2 Antibody Across Mouse Tissues and Cell Lines WB. Western blot testing with VEGF Receptor 2 Antibody. Lane 1: heart; lane 2: thymus; lane 3: spleen; lane 4: kidney; lane 5: brain; lane 6: lung; lane 7: HEPA1-6 cells; lane 8: NIH 3T3 cells. Multiple VEGFR2-reactive species are visible, including high-molecular-weight bands in the approximately 150-230 kDa region. The page lists approximately 152 kDa for immature, 180-200 kDa for intermediate and 220-230 kDa for mature VEGFR2, consistent with extensive receptor processing and glycosylation. This multi-sample experiment supports western blot detection with the VEGFR2 Polyclonal Antibody.

VEGF Receptor 2 Antibody in NIH 3T3 Cells IF. Immunofluorescence staining of FFPE NIH 3T3 cells with VEGF Receptor 2 Antibody and DAPI nuclear counterstain. HIER was performed by steaming in pH 6 citrate buffer for 20 min. Red VEGFR2 immunofluorescence is distributed through the cell bodies and processes surrounding the blue nuclei, producing clear cellular labeling with comparatively low background. The staining demonstrates cellular detection with the VEGFR2 Polyclonal Antibody.

VEGF Receptor 2 Antibody in MCF7 Cells FACS. Flow cytometry analysis of MCF7 cells with VEGF Receptor 2 Antibody at 1 ug per million cells after blocking with goat sera. Cells alone are shown in red, the isotype control in green and VEGF Receptor 2 Antibody staining in blue. The blue population shows a pronounced rightward fluorescence shift relative to both controls, demonstrating clear antibody-dependent detection of VEGFR2 by flow cytometry with the VEGFR2 Polyclonal Antibody.

VEGF Receptor 2 Antibody in LLC1 Cells FACS. Flow cytometry analysis of LLC1 cells with VEGF Receptor 2 Antibody at 1 ug per million cells after blocking with goat sera. Cells alone are shown in red, the isotype control in green and VEGF Receptor 2 Antibody staining in blue. A substantial rightward shift of the blue population relative to the controls demonstrates antibody-dependent detection of VEGFR2 and provides additional flow cytometry validation for the VEGFR2 Polyclonal Antibody.