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Home >> Antibodies >> VEGF Antibody

VEGF Antibody [clone VG1] (V8154)

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  Catalog No Formulation Size Price (USD)  
Image V8154-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8154-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8154SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human tonsil with VEGF antibody (clone VG1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil with VEGF antibody (clone VG1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free VEGF antibody (clone VG1) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name VG1
Purity Protein G affinity chromatography
UniProt P15692
Applications ELISA (order BSA-free format for coating) :
Immunohistochemistry (FFPE) : 2-4ug/ml
Limitations This VEGF antibody is available for research use only.
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Description

This mAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet-derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Application Notes

Optimal dilution of the VEGF antibody should be determined by the researcher.

Immunogen

Recombinant human protein was used as the immunogen for the VEGF antibody.

Storage

Store the VEGF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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