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Home >> Antibodies >> VEGF Antibody / VEGFA

VEGF Antibody / VEGFA (R30265)

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  Catalog No Formulation Size Price (USD)  
Image R30265 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of VEGF antibody and Lane 1: Recombinant human protein 10ng; 2: 5ng; 3: 2.5ng
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Western blot testing of VEGF antibody and Lane 1: Recombinant human protein 10ng; 2: 5ng; 3: 2.5ng
IHC-P: VEGF antibody testing of human lung cancer tissue
Western blot testing of human placenta lysate (50ug/lane) with VEGF antibody at 1ug/ml. Expected molecular weight 19~22 kDa (monomer) and 38~44 kDa (dimer). Banding may appear larger than predicted due to glycosylation.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% thimerosal and 0.005% sodium azide
UniProt P15692
Localization Cytoplasmic, cell surface and extracellular (secreted)
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Limitations This VEGF antibody is available for research use only.
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Description

VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. Vascular endothelial growth factor is a mitogen primarily for vascular endothelial cells. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. Vascular endothelial growth factor (VEGF) induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism. Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo.

Application Notes

The stated application concentrations are suggested starting points. Titration of the VEGF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the N-terminus of human VEGFA (VDIFQEYPDEIEYIFK) was used as the immunogen for this VEGF antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the VEGF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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