VDBP Antibody / Endocrine Vitamin D Transport Protein Antibody [clone VDBP/4481] (V9503)

VDBP Antibody Pancreatic Islet IHC. Immunohistochemistry analysis of FFPE human pancreatic tissue stained with VDBP Antibody clone VDBP/4481 demonstrates strong cytoplasmic HRP-DAB brown staining within islet-associated endocrine cell populations, consistent with the role of Vitamin D binding protein / VDBP in endocrine metabolism, circulating transport physiology, and vitamin D-associated regulatory pathways. The concentrated staining pattern within pancreatic endocrine structures aligns with the biologic importance of vitamin D-associated signaling in metabolic homeostasis and endocrine tissue function. Tissue sections were stained using 2 ug/ml antibody in PBS for 30 min at room temperature. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

VDBP Antibody Renal Oncocytoma IHC. Immunohistochemistry analysis of FFPE human renal oncocytoma tissue stained with VDBP Antibody clone VDBP/4481 demonstrates strong granular cytoplasmic HRP-DAB brown staining within tumor-associated epithelial cell populations, consistent with the role of Vitamin D binding protein / VDBP in endocrine transport physiology and metabolic carrier-associated signaling pathways. The observed staining profile aligns with the secretory and transport-associated biology of this circulating vitamin D metabolite carrier protein. Tissue sections were stained using 2 ug/ml antibody in PBS for 30 min at room temperature. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free VDBP antibody (clone VDBP/4481) as confirmation of integrity and purity.

VDBP Antibody Microarray Specificity Validation. Analysis of a HuProt(TM) human protein microarray containing more than 19,000 full-length human proteins using clone VDBP/4481 demonstrates highly selective binding to Vitamin D binding protein / GC with minimal off-target reactivity detected across the tested human proteome. The strong enrichment of VDBP signal relative to lower-ranking proteins supports the specificity of this monoclonal antibody for studies involving endocrine transport biology, circulating carrier proteins, vitamin D metabolism, and systemic metabolic homeostasis pathways.