VCP Antibody / Transitional endoplasmic reticulum ATPase (R31778)

IHC staining of FFPE human glioma tissue with VCP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human glioma tissue with VCP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human meningioma tissue with VCP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse brain tissue with VCP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat brain tissue with VCP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of frozen mouse brain tissue with VCP antibody.

IHC staining of frozen rat brain tissue with VCP antibody.

Immunofluorescent staining of FFPE human A431 cells with VCP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) HeLa, 2) A431, 3) U-87 MG, 4) A549, 5) SH-SY5Y, 6) K562, 7) Raji, 8) HepG2 and rat 9) heart, 10) spleen, 11) kidney, 12) liver and mouse 13) heart, 14) kidney, 15) liver and 16) HEPA1-6 cell lysate with VCP antibody. Predicted molecular weight ~89 kDa.

Flow cytometry testing of human HepG2 cells with VCP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VCP antibody.