VAPA Antibody | ER Membrane Contact Site Protein

	Catalog No	Formulation	Size	Price (USD)
	RQ7451	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

VAPA Antibody Cervical Cancer IHC. Immunohistochemistry staining of FFPE human cervical cancer tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates moderate to strong HRP-DAB brown staining throughout malignant epithelial cell populations. The observed staining pattern is predominantly membranous and cytoplasmic, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, and organelle communication. Staining highlights tumor-associated epithelial structures and supports evaluation of VAPA expression and localization in human cervical cancer tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

VAPA Antibody Lung Adenocarcinoma IHC. Immunohistochemistry staining of FFPE human lung adenocarcinoma tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates strong HRP-DAB brown staining within malignant glandular epithelial cells. The observed staining pattern is predominantly membranous and cytoplasmic, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Staining prominently outlines neoplastic glandular structures and supports evaluation of VAPA expression and localization in human lung adenocarcinoma tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

VAPA Antibody Colorectal Adenocarcinoma IHC. Immunohistochemistry staining of FFPE human colorectal adenocarcinoma tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates strong HRP-DAB brown staining throughout malignant glandular epithelial cell populations. The observed staining pattern is predominantly membranous and cytoplasmic, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Staining prominently highlights neoplastic glandular structures and supports evaluation of VAPA expression and localization in human colorectal adenocarcinoma tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

VAPA Antibody Placenta IHC. Immunohistochemistry staining of FFPE human placenta tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates distinct HRP-DAB brown staining within trophoblastic epithelial cell populations lining placental villi. The observed staining pattern is predominantly membranous with associated cytoplasmic signal, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Staining highlights placental villous architecture and supports evaluation of VAPA expression and localization in human placental tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

VAPA Antibody Mouse Colon IHC. Immunohistochemistry staining of FFPE mouse colon tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates moderate to strong HRP-DAB brown staining within colonic epithelial cells lining the mucosal crypt structures. The observed staining pattern is predominantly membranous and cytoplasmic, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Staining highlights epithelial architecture throughout the colonic mucosa and supports evaluation of VAPA expression and localization in mouse gastrointestinal tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

VAPA Antibody Rat Colon IHC. Immunohistochemistry staining of FFPE rat colon tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates moderate HRP-DAB brown staining within colonic epithelial cells and associated glandular structures. The observed staining pattern is predominantly membranous and cytoplasmic, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Staining highlights epithelial cell populations throughout the colonic mucosa and supports evaluation of VAPA expression and localization in rat gastrointestinal tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

VAPA Antibody Colorectal Adenocarcinoma IF. Immunofluorescence staining of FFPE human colorectal adenocarcinoma tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates strong red fluorescence throughout malignant glandular epithelial cell populations. The observed staining pattern is predominantly cytoplasmic with associated membranous localization, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Signal highlights neoplastic glandular structures within the tumor tissue, while nuclei are counterstained with DAPI (blue). Heat-induced epitope retrieval was performed by steaming tissue sections in pH 8 EDTA buffer for 20 minutes prior to staining. Target protein is visualized in red and nuclei are shown in blue.

VAPA Antibody Rat Colon IF. Immunofluorescence staining of FFPE rat colon tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates distinct red fluorescence within colonic epithelial cell populations lining the mucosal glandular structures. The observed staining pattern is predominantly cytoplasmic with associated membranous localization, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Signal highlights epithelial architecture throughout the colonic mucosa, while nuclei are counterstained with DAPI (blue). Heat-induced epitope retrieval was performed by steaming tissue sections in pH 8 EDTA buffer for 20 minutes prior to staining. Target protein is visualized in red and nuclei are shown in blue.

VAPA Antibody PC-3 IF. Immunofluorescence staining of FFPE human PC-3 cells using VAPA Antibody / ER Membrane Contact Site Protein demonstrates distinct cytoplasmic green fluorescence consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Signal is distributed throughout the cytoplasm with a reticular appearance characteristic of endoplasmic reticulum-associated proteins, while nuclei are counterstained with DAPI (blue). Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to staining. Target protein is visualized in green and nuclei are shown in blue.

VAPA Antibody Multi-Species WB. Western blot analysis of 1) human HeLa, 2) human K562, 3) human 293T, 4) human HepG2, 5) rat brain, and 6) mouse brain lysates using VAPA Antibody / ER Membrane Contact Site Protein demonstrates a prominent band at approximately 28-33 kDa, consistent with the expected molecular weight of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA). Multiple closely migrating bands are observed in some samples, consistent with reported VAPA isoforms and post-translationally modified forms. VAPA is an endoplasmic reticulum-associated membrane protein that regulates membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. The observed cross-species reactivity supports application of this antibody for detection of VAPA expression in human, rat, and mouse tissues and cell lines.

VAPA Antibody U-87 MG FACS. Flow cytometry analysis of human U-87 MG cells using VAPA Antibody / ER Membrane Contact Site Protein demonstrates a clear positive fluorescence shift relative to both unstained cells and isotype control-treated cells, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA). Overlay histograms compare cells alone (red), isotype control antibody (green), and VAPA antibody (blue), with the specific antibody producing a pronounced rightward shift indicative of target-associated binding. VAPA is an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Cells were blocked with goat serum prior to staining, and antibody was used at 1 Î¼g per million cells. These results support application of the antibody for flow cytometric detection of VAPA expression in human cell populations.

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VAPA Antibody Cervical Cancer IHC. Immunohistochemistry staining of FFPE human cervical cancer tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates moderate to strong HRP-DAB brown staining throughout malignant epithelial cell populations. The observed staining pattern is predominantly membranous and cytoplasmic, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, and organelle communication. Staining highlights tumor-associated epithelial structures and supports evaluation of VAPA expression and localization in human cervical cancer tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

VAPA Antibody Lung Adenocarcinoma IHC. Immunohistochemistry staining of FFPE human lung adenocarcinoma tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates strong HRP-DAB brown staining within malignant glandular epithelial cells. The observed staining pattern is predominantly membranous and cytoplasmic, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Staining prominently outlines neoplastic glandular structures and supports evaluation of VAPA expression and localization in human lung adenocarcinoma tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

VAPA Antibody Colorectal Adenocarcinoma IHC. Immunohistochemistry staining of FFPE human colorectal adenocarcinoma tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates strong HRP-DAB brown staining throughout malignant glandular epithelial cell populations. The observed staining pattern is predominantly membranous and cytoplasmic, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Staining prominently highlights neoplastic glandular structures and supports evaluation of VAPA expression and localization in human colorectal adenocarcinoma tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

VAPA Antibody Placenta IHC. Immunohistochemistry staining of FFPE human placenta tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates distinct HRP-DAB brown staining within trophoblastic epithelial cell populations lining placental villi. The observed staining pattern is predominantly membranous with associated cytoplasmic signal, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Staining highlights placental villous architecture and supports evaluation of VAPA expression and localization in human placental tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

VAPA Antibody Mouse Colon IHC. Immunohistochemistry staining of FFPE mouse colon tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates moderate to strong HRP-DAB brown staining within colonic epithelial cells lining the mucosal crypt structures. The observed staining pattern is predominantly membranous and cytoplasmic, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Staining highlights epithelial architecture throughout the colonic mucosa and supports evaluation of VAPA expression and localization in mouse gastrointestinal tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

VAPA Antibody Rat Colon IHC. Immunohistochemistry staining of FFPE rat colon tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates moderate HRP-DAB brown staining within colonic epithelial cells and associated glandular structures. The observed staining pattern is predominantly membranous and cytoplasmic, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Staining highlights epithelial cell populations throughout the colonic mucosa and supports evaluation of VAPA expression and localization in rat gastrointestinal tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

VAPA Antibody Colorectal Adenocarcinoma IF. Immunofluorescence staining of FFPE human colorectal adenocarcinoma tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates strong red fluorescence throughout malignant glandular epithelial cell populations. The observed staining pattern is predominantly cytoplasmic with associated membranous localization, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Signal highlights neoplastic glandular structures within the tumor tissue, while nuclei are counterstained with DAPI (blue). Heat-induced epitope retrieval was performed by steaming tissue sections in pH 8 EDTA buffer for 20 minutes prior to staining. Target protein is visualized in red and nuclei are shown in blue.

VAPA Antibody Rat Colon IF. Immunofluorescence staining of FFPE rat colon tissue using VAPA Antibody / ER Membrane Contact Site Protein demonstrates distinct red fluorescence within colonic epithelial cell populations lining the mucosal glandular structures. The observed staining pattern is predominantly cytoplasmic with associated membranous localization, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Signal highlights epithelial architecture throughout the colonic mucosa, while nuclei are counterstained with DAPI (blue). Heat-induced epitope retrieval was performed by steaming tissue sections in pH 8 EDTA buffer for 20 minutes prior to staining. Target protein is visualized in red and nuclei are shown in blue.

VAPA Antibody PC-3 IF. Immunofluorescence staining of FFPE human PC-3 cells using VAPA Antibody / ER Membrane Contact Site Protein demonstrates distinct cytoplasmic green fluorescence consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Signal is distributed throughout the cytoplasm with a reticular appearance characteristic of endoplasmic reticulum-associated proteins, while nuclei are counterstained with DAPI (blue). Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to staining. Target protein is visualized in green and nuclei are shown in blue.

VAPA Antibody Multi-Species WB. Western blot analysis of 1) human HeLa, 2) human K562, 3) human 293T, 4) human HepG2, 5) rat brain, and 6) mouse brain lysates using VAPA Antibody / ER Membrane Contact Site Protein demonstrates a prominent band at approximately 28-33 kDa, consistent with the expected molecular weight of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA). Multiple closely migrating bands are observed in some samples, consistent with reported VAPA isoforms and post-translationally modified forms. VAPA is an endoplasmic reticulum-associated membrane protein that regulates membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. The observed cross-species reactivity supports application of this antibody for detection of VAPA expression in human, rat, and mouse tissues and cell lines.

VAPA Antibody U-87 MG FACS. Flow cytometry analysis of human U-87 MG cells using VAPA Antibody / ER Membrane Contact Site Protein demonstrates a clear positive fluorescence shift relative to both unstained cells and isotype control-treated cells, consistent with expression of Vesicle-Associated Membrane Protein-Associated Protein A (VAPA). Overlay histograms compare cells alone (red), isotype control antibody (green), and VAPA antibody (blue), with the specific antibody producing a pronounced rightward shift indicative of target-associated binding. VAPA is an endoplasmic reticulum-associated protein involved in membrane contact site formation, intracellular trafficking, lipid transport, and organelle communication. Cells were blocked with goat serum prior to staining, and antibody was used at 1 μg per million cells. These results support application of the antibody for flow cytometric detection of VAPA expression in human cell populations.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	Q9P0L0
Localization	Cell surface, cytoplasmic, nuclear membrane
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells
Limitations	This VAPA Antibody / ER Membrane Contact Site Protein is available for research use only.
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Description

VAPA antibody recognizes Vesicle-Associated Membrane Protein-Associated Protein A (VAPA), a highly conserved endoplasmic reticulum membrane protein that functions as a central organizer of intracellular membrane contact sites. VAPA is widely expressed in mammalian tissues and plays a critical role in coordinating communication between the endoplasmic reticulum and numerous cellular organelles including the Golgi apparatus, endosomes, lysosomes, mitochondria, lipid droplets, and plasma membrane. Through these interactions, VAPA regulates intracellular trafficking, membrane organization, lipid transport, and maintenance of cellular homeostasis. Its broad involvement in membrane-associated processes has established VAPA as an important target for studies of cell biology, organelle communication, and membrane dynamics.

VAPA belongs to a family of membrane-associated proteins that serve as molecular scaffolds linking intracellular organelles through specialized membrane contact sites. These contact regions permit direct exchange of lipids, metabolites, calcium ions, and signaling molecules without membrane fusion. VAPA mediates many of these functions through interactions with proteins containing FFAT (two phenylalanines in an acidic tract) motifs, allowing recruitment of transport proteins and regulatory complexes to endoplasmic reticulum membranes. As a result, VAPA contributes to cholesterol trafficking, phospholipid distribution, calcium signaling, and preservation of endoplasmic reticulum architecture. Researchers frequently utilize VAPA antibody reagents to investigate the structural organization of intracellular membrane networks and the mechanisms governing organelle communication.

In addition to its role in membrane organization, VAPA participates in pathways associated with vesicular trafficking, autophagy, stress responses, and secretory transport. Proper coordination of these pathways is essential for normal cellular physiology, and disruption of VAPA-regulated functions has been linked to a variety of pathological conditions. Altered VAPA expression or activity has been associated with neurodegenerative disease, cancer progression, viral infection, and disorders involving endoplasmic reticulum dysfunction. Several viruses utilize VAPA-containing membrane contact sites to support replication, while cancer cells may exploit VAPA-dependent trafficking pathways to maintain growth and adaptation under cellular stress. These observations have generated increasing interest in VAPA as a marker of intracellular transport systems and membrane remodeling processes.

One of the major differentiating features of VAPA compared with many conventional membrane trafficking proteins is its direct involvement in endoplasmic reticulum-organelle communication. Rather than functioning solely within vesicular transport pathways, VAPA helps establish physical and functional connectivity between intracellular compartments. This unique role makes VAPA antibody reagents particularly valuable for studies examining membrane contact site biology, lipid homeostasis, organelle organization, and intracellular signaling. Investigators studying mitochondrial function, lipid metabolism, calcium regulation, and ER-associated cellular processes frequently evaluate VAPA expression as part of broader analyses of cellular architecture and homeostatic regulation.

VAPA antibody is suitable for investigations of endoplasmic reticulum biology, intracellular trafficking, membrane contact sites, lipid transport pathways, organelle communication networks, and cellular stress responses. Common search terms including VAPA antibody, Vesicle-Associated Membrane Protein-Associated Protein A antibody, ER membrane protein antibody, membrane contact site protein antibody, intracellular trafficking antibody, lipid transport protein antibody, and organelle communication marker antibody reflect the diverse research applications associated with this target. Because VAPA occupies a central position at the intersection of membrane organization and intracellular signaling, it remains an important protein for studies spanning cell biology, neuroscience, cancer research, virology, and metabolism.

NSJ Bioreagents offers VAPA antibody reagents to support investigations of membrane dynamics, endoplasmic reticulum organization, intracellular transport mechanisms, lipid homeostasis, and organelle communication. By enabling detection of this essential membrane-organizing protein, VAPA antibody products provide researchers with valuable tools for exploring the molecular pathways that maintain cellular architecture, coordinate intracellular signaling, and regulate communication between organelles.

Researchers studying intracellular trafficking, membrane contact sites, organelle communication, and endoplasmic reticulum function may also be interested in our Cell Biology Antibodies page featuring proteins involved in cellular organization, transport pathways, and membrane dynamics.

Application Notes

Optimal dilution of the VAPA Antibody / ER Membrane Contact Site Protein should be determined by the researcher.

Immunogen

Amino acids KHEQILVLDPPTDLKFK from the human protein were used as the immunogen for the VAPA antibody.

Storage

After reconstitution, the VAPA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

VAPA Antibody, Vesicle-Associated Membrane Protein-Associated Protein A Antibody, ER Membrane Contact Site Protein Antibody, Endoplasmic Reticulum Membrane Protein Antibody, Intracellular Trafficking Protein Antibody, Organelle Communication Protein Antibody, Lipid Transport Protein Antibody, Membrane Contact Site Marker Antibody

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VAPA Antibody / ER Membrane Contact Site Protein (RQ7451)

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