Uroplakin 3B Antibody / UPK3B [clone UPK3B/8550R] (V4629)

Immunohistochemistry of Uroplakin 3B antibody in human bladder tissue. Clone UPK3B/8550R demonstrates crisp membranous HRP-DAB brown staining along the apical surface of urothelial umbrella cells lining the bladder lumen. Staining highlights the specialized luminal membrane of differentiated superficial urothelial cells, with strong signal concentrated at the cell borders facing the bladder cavity. Underlying intermediate and basal urothelial layers show minimal to absent staining, and adjacent stromal components remain largely negative, providing clear contrast with the positive epithelial surface. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human bladder tissue with Uroplakin 3B antibody (clone UPK3B/8550R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.