Uroplakin 1B Antibody / Urothelial Apical Surface Protein Antibody [clone UPK1B/3273] (V8153)

Uroplakin 1B Antibody. Immunohistochemistry analysis of Uroplakin 1B / UPK1B antibody in human urothelial carcinoma tissue using clone UPK1B/3273. FFPE sections show strong HRP-DAB brown membranous staining in tumor epithelial cells with accentuation along the luminal border, consistent with apical surface localization in urothelial cells. This Urothelial Apical Surface Protein Antibody highlights the polarized distribution of UPK1B at the apical membrane of urothelial tumor cells. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to staining.

Uroplakin 1B Antibody. Immunohistochemistry analysis of Uroplakin 1B / UPK1B antibody in human urothelial carcinoma tissue using clone UPK1B/3273. FFPE sections demonstrate strong HRP-DAB brown membranous staining in tumor epithelial cells with clear apical surface accentuation, outlining luminal-facing cell borders consistent with polarized urothelial architecture. This Urothelial Apical Surface Protein Antibody highlights the apical membrane localization and surface-oriented distribution of UPK1B in urothelial tumor cells. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to staining.

Uroplakin 1B Antibody. Immunohistochemistry analysis of Uroplakin 1B / UPK1B antibody in human urothelial carcinoma tissue using clone UPK1B/3273. FFPE sections show diffuse HRP-DAB brown membranous staining in tumor epithelial cells with partial luminal surface accentuation, consistent with apical membrane localization in urothelial cells. This Urothelial Apical Surface Protein Antibody highlights the polarized cell surface distribution of UPK1B within urothelial tumor epithelium. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to staining.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Uroplakin 1B antibody (clone UPK1B/3273). These results demonstrate the foremost specificity of the UPK1B/3273 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free UPK1B antibody as confirmation of integrity and purity.