Uroplakin 1A Antibody Microarray Validated / UPK1A [clone UPK1A/2925] (V7937)

Immunohistochemistry of Uroplakin 1A antibody in human urothelial carcinoma. Formalin-fixed, paraffin-embedded human urothelial carcinoma tissue was stained with microarray validated Uroplakin 1A antibody (clone UPK1A/2925) following heat-induced epitope retrieval by boiling in pH 9 10 mM Tris with 1 mM EDTA for 10-20 minutes and cooling prior to testing. HRP-DAB brown chromogenic signal demonstrates strong membranous staining in tumor cells, consistent with apical membrane localization and urothelial differentiation associated with Uroplakin 1A, while surrounding stromal cells show minimal background staining.

Immunohistochemistry of Uroplakin 1A antibody in human urothelial carcinoma. Formalin-fixed, paraffin-embedded human urothelial carcinoma tissue was stained with microarray validated Uroplakin 1A antibody (clone UPK1A/2925) following heat-induced epitope retrieval by boiling in pH 9 10 mM Tris with 1 mM EDTA for 10-20 minutes and cooling prior to testing. HRP-DAB brown chromogenic signal highlights prominent membranous staining in malignant urothelial cells, consistent with the apical membrane localization and differentiation-associated expression pattern of Uroplakin 1A, while adjacent stromal elements remain largely negative.

HuProt protein microarray specificity analysis of Uroplakin 1A antibody (clone UPK1A/2925). The antibody was evaluated against a HuProt array containing more than 19,000 full-length human proteins. Signal intensity is expressed as a Z-score, representing the number of standard deviations above the mean signal generated across the array when the antibody, together with a fluorescently labeled anti-IgG secondary antibody, binds to a given protein. Proteins are ranked in descending order by Z-score, and the S-score reflects the difference in Z-scores between the top-ranked protein and the next highest signal, providing a quantitative measure of relative target specificity. Clone UPK1A/2925 demonstrates a markedly elevated Z-score for Uroplakin 1A with strong S-score separation from other proteins, supporting highly specific recognition of UPK1A on the array and confirming its microarray validated status.

SDS-PAGE analysis of purified, BSA-free Uroplakin 1A antibody (clone UPK1A/2925) as confirmation of integrity and purity.