UQCRC2 Antibody | FY13603 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	FY13603	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

UQCRC2 Antibody by IF in HeLa cells. Immunofluorescence staining of HeLa cells using Ubiquinol-cytochrome c reductase core protein 2 Antibody reveals red punctate and reticular cytoplasmic staining around DAPI-positive nuclei. Nuclei were counterstained with DAPI (blue). The observed distribution is described from the fluorescence image without claiming organelle-specific colocalization in the absence of an appropriate marker.

UQCRC2 Antibody by WB in human and rodent samples. Western blot analysis using Ubiquinol-cytochrome c reductase core protein 2 Antibody detects a prominent immunoreactive band near 48 kDa in HepG2 (lane 1), K562 (lane 2), rat heart (lane 3), rat kidney (lane 4), mouse heart (lane 5), mouse kidney (lane 6), and mouse colon (lane 7). The observed migration is compared with the calculated molecular weight of approximately 48 kDa. Reduced lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.1 ug/mL overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection.

UQCRC2 Antibody by IHC in human gastric carcinoma. Immunohistochemistry of paraffin-embedded human gastric carcinoma using Ubiquinol-cytochrome c reductase core protein 2 Antibody reveals brown cytoplasmic staining in malignant glandular epithelium. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), blocking with goat serum, and primary antibody incubation at 2 ug/mL overnight at 4oC. Bound antibody was detected using an HRP-conjugated secondary antibody and DAB chromogen.

UQCRC2 Antibody by IHC in mouse kidney. Immunohistochemistry of paraffin-embedded mouse kidney using Ubiquinol-cytochrome c reductase core protein 2 Antibody reveals brown staining in renal tubular epithelium. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), blocking with goat serum, and primary antibody incubation at 2 ug/mL overnight at 4oC. Bound antibody was detected using an HRP-conjugated secondary antibody and DAB chromogen.

UQCRC2 Antibody by IHC in rat kidney. Immunohistochemistry of paraffin-embedded rat kidney using Ubiquinol-cytochrome c reductase core protein 2 Antibody reveals brown cytoplasmic staining in renal tubules. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), blocking with goat serum, and primary antibody incubation at 2 ug/mL overnight at 4oC. Bound antibody was detected using an HRP-conjugated secondary antibody and DAB chromogen.

UQCRC2 Antibody by IP in HeLa cells. Immunoprecipitation of HeLa whole-cell lysate using Ubiquinol-cytochrome c reductase core protein 2 Antibody was evaluated by Western blot. Lane 1 contains 30 ug input lysate, lane 2 contains a rabbit IgG control immunoprecipitate, and lane 3 contains the specific antibody immunoprecipitate prepared from 500 ug lysate with 2 ug antibody. The prominent band near 48 kDa in the input and specific immunoprecipitate is consistent with the expected UQCRC2 molecular weight. Detection used a rabbit primary antibody and HRP-conjugated secondary antibody with chemiluminescence.

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UQCRC2 Antibody by IF in HeLa cells. Immunofluorescence staining of HeLa cells using Ubiquinol-cytochrome c reductase core protein 2 Antibody reveals red punctate and reticular cytoplasmic staining around DAPI-positive nuclei. Nuclei were counterstained with DAPI (blue). The observed distribution is described from the fluorescence image without claiming organelle-specific colocalization in the absence of an appropriate marker.

UQCRC2 Antibody by WB in human and rodent samples. Western blot analysis using Ubiquinol-cytochrome c reductase core protein 2 Antibody detects a prominent immunoreactive band near 48 kDa in HepG2 (lane 1), K562 (lane 2), rat heart (lane 3), rat kidney (lane 4), mouse heart (lane 5), mouse kidney (lane 6), and mouse colon (lane 7). The observed migration is compared with the calculated molecular weight of approximately 48 kDa. Reduced lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.1 ug/mL overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection.

UQCRC2 Antibody by IHC in human gastric carcinoma. Immunohistochemistry of paraffin-embedded human gastric carcinoma using Ubiquinol-cytochrome c reductase core protein 2 Antibody reveals brown cytoplasmic staining in malignant glandular epithelium. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), blocking with goat serum, and primary antibody incubation at 2 ug/mL overnight at 4oC. Bound antibody was detected using an HRP-conjugated secondary antibody and DAB chromogen.

UQCRC2 Antibody by IHC in human gastric carcinoma. Immunohistochemistry of paraffin-embedded human gastric carcinoma using Ubiquinol-cytochrome c reductase core protein 2 Antibody reveals granular brown staining within epithelial tumor glands. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), blocking with goat serum, and primary antibody incubation at 2 ug/mL overnight at 4oC. Bound antibody was detected using an HRP-conjugated secondary antibody and DAB chromogen.

UQCRC2 Antibody by IHC in mouse kidney. Immunohistochemistry of paraffin-embedded mouse kidney using Ubiquinol-cytochrome c reductase core protein 2 Antibody reveals brown staining in renal tubular epithelium. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), blocking with goat serum, and primary antibody incubation at 2 ug/mL overnight at 4oC. Bound antibody was detected using an HRP-conjugated secondary antibody and DAB chromogen.

UQCRC2 Antibody by IHC in rat kidney. Immunohistochemistry of paraffin-embedded rat kidney using Ubiquinol-cytochrome c reductase core protein 2 Antibody reveals brown cytoplasmic staining in renal tubules. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), blocking with goat serum, and primary antibody incubation at 2 ug/mL overnight at 4oC. Bound antibody was detected using an HRP-conjugated secondary antibody and DAB chromogen.

UQCRC2 Antibody by IP in HeLa cells. Immunoprecipitation of HeLa whole-cell lysate using Ubiquinol-cytochrome c reductase core protein 2 Antibody was evaluated by Western blot. Lane 1 contains 30 ug input lysate, lane 2 contains a rabbit IgG control immunoprecipitate, and lane 3 contains the specific antibody immunoprecipitate prepared from 500 ug lysate with 2 ug antibody. The prominent band near 48 kDa in the input and specific immunoprecipitate is consistent with the expected UQCRC2 molecular weight. Detection used a rabbit primary antibody and HRP-conjugated secondary antibody with chemiluminescence.

Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit Ig
Buffer	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
UniProt	P22695
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Flow Cytometry : 1-3ug/million cells ELISA : 0.1-0.5 ug/ml
Limitations	The UQCRC2 Antibody / Ubiquinol-cytochrome c reductase core protein 2 Antibody is available for research use only.
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Description

UQCRC2 Antibody / Ubiquinol-cytochrome c reductase core protein 2 Antibody detects UQCRC2, a component of mitochondrial respiratory chain complex III, also called the cytochrome bc1 complex. Complex III transfers electrons from reduced ubiquinone to cytochrome c as part of oxidative phosphorylation. This process is coupled to proton movement across the inner mitochondrial membrane through the Q cycle, contributing to the electrochemical gradient used by ATP synthase. UQCRC2 is one of the core subunits that support the organization of the assembled enzyme.

Complex III contains catalytic subunits responsible for electron transfer as well as additional proteins that stabilize the complex and assist its assembly. UQCRC2 is not itself the cytochrome that carries electrons between redox centers. Instead, it forms part of the structural framework that helps maintain the integrity of the respiratory complex. Complex III can also participate in larger assemblies with other respiratory chain complexes, sometimes described as supercomplexes. Such organization may influence respiratory chain stability and the arrangement of electron-transfer components. The designation Ubiquinol-cytochrome c reductase core protein 2 Antibody refers to the same gene product.

Dysfunction of complex III can impair mitochondrial ATP production and alter the generation of reactive oxygen species. The consequences depend on the specific subunit affected, the degree of residual respiratory activity, and cellular energy demands. UQCRC2 abundance is frequently considered in the context of respiratory complex composition, but protein levels alone do not establish enzymatic activity. NSJ Bioreagents includes UQCRC2 among mitochondrial proteins involved in the structural organization of oxidative phosphorylation and the maintenance of respiratory chain function. A UQCRC2 Antibody can help contextualize the molecular functions and regulation of this protein.

Explore related research targets in our Metabolism Antibody / Metabolism Antibodies collection.

Application Notes

The ideal working dilution for the UQCRC2 Antibody / Ubiquinol-cytochrome c reductase core protein 2 Antibody should be established by the investigator based on assay conditions.

Immunogen

The UQCRC2 Antibody was generated using e. coli-derived human UQCRC2 recombinant protein (amino acids L52-K436).

Storage

The UQCRC2 Antibody is stable for up to one month at 4oC after reconstitution. For longer-term storage, aliquot and store at -20oC for up to six months. Do not repeatedly freeze and thaw.

Alternate Names

UQCRC2 antibody, Cytochrome b-c1 complex subunit 2, mitochondrial antibody, Cytochrome b-c1 complex subunit 2 antibody, mitochondrial antibody, ubiquinol-cytochrome c reductase core protein 2 antibody, UQCR2 antibody, Ubiquinol-cytochrome-c reductase complex core protein 2 antibody, QCR2 antibody, Core protein II antibody

UQCRC2 Antibody / Ubiquinol-cytochrome c reductase core protein 2 Antibody (FY13603)

Description

Application Notes

Immunogen

Storage

Alternate Names

Area of Research

Product Type

Protocols

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NSJ Bioreagents