UPK3B Antibody / Uroplakin 3B [clone UPK3B/3270] (V4622)

Immunohistochemistry of UPK3B antibody in human bladder tissue. The mouse monoclonal clone UPK3B/3270 demonstrates distinct membranous HRP-DAB brown staining along the apical surface of urothelial umbrella cells, consistent with localization of Uroplakin 3B to differentiated epithelial cell membranes. Staining is concentrated at the luminal interface, highlighting the specialized surface layer of the urothelium, while underlying basal and intermediate cell layers show minimal to absent signal. Surrounding stromal cells remain largely negative, providing clear contrast with positive urothelial elements. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

Immunohistochemistry of UPK3B antibody in human bladder tissue. The mouse monoclonal clone UPK3B/3270 shows crisp membranous HRP-DAB brown staining along the apical surface of urothelial umbrella cells lining the bladder lumen. The staining pattern highlights the luminal cell borders and specialized apical membranes, consistent with localization of Uroplakin 3B in differentiated superficial urothelial cells. Basal and intermediate cell layers display little to no specific staining, and adjacent stromal components are largely negative, providing clear contrast with the positively labeled surface epithelium. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using UPK3B antibody (clone UPK3B/3270) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

SDS-PAGE analysis of purified, BSA-free UPK3B antibody (clone UPK3B/3270) as confirmation of integrity and purity.