UPK1A Antibody Microarray Validated / Uroplakin 1A [clone UPK1A/2921] (V7671)

Immunohistochemistry of UPK1A antibody in human urothelial carcinoma. Formalin-fixed, paraffin-embedded human urothelial carcinoma tissue was stained with microarray validated Uroplakin 1A antibody (clone UPK1A/2921) following heat-induced epitope retrieval by boiling in pH 9 10 mM Tris with 1 mM EDTA for 10-20 minutes and cooling prior to testing. HRP-DAB brown chromogenic signal demonstrates strong membranous and cytoplasmic staining in malignant urothelial cells, consistent with urothelial differentiation and the characteristic apical membrane-associated expression pattern of Uroplakin 1A, while surrounding stromal elements remain largely negative.

IHC staining of FFPE human urothelial carcinoma with UPK1A antibody (clone UPK1A/2921). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Immunohistochemistry of UPK1A antibody in human urinary bladder. Formalin-fixed, paraffin-embedded human urinary bladder tissue was stained with microarray validated Uroplakin 1A antibody (clone UPK1A/2921) following heat-induced epitope retrieval by boiling in pH 9 10 mM Tris with 1 mM EDTA for 10-20 minutes and cooling prior to testing. HRP-DAB brown chromogenic signal highlights strong membranous staining of urothelial umbrella cells along the luminal surface, consistent with the apical membrane localization and differentiation-associated expression pattern of Uroplakin 1A, while underlying stromal tissue shows minimal background staining.

HuProt protein microarray specificity analysis of UPK1A antibody (clone UPK1A/2921). The antibody was screened against a HuProt microarray containing more than 19,000 full-length human proteins. Signal intensity is expressed as a Z-score, representing the number of standard deviations above the mean signal generated across the array when the antibody, together with a fluorescently labeled anti-IgG secondary antibody, binds to a specific protein. Proteins are ranked in descending order by Z-score, and the S-score reflects the difference in Z-scores between the top-ranked protein and the next highest signal, providing a quantitative measure of relative target specificity. Clone UPK1A/2921 demonstrates a markedly elevated Z-score for Uroplakin 1A with strong S-score separation from other proteins, supporting highly specific recognition of UPK1A on the array and confirming its microarray validated status.

SDS-PAGE analysis of purified, BSA-free UPK1A antibody (clone UPK1A/2921) as confirmation of integrity and purity.