UPF1 Antibody / RENT1 (R32296)

Immunofluorescence analysis of UPF1 / RENT1 antibody in human A431 cells. FFPE human A431 cells show cytoplasmic green fluorescence consistent with UPF1 expression, while nuclei are counterstained with DAPI (blue). The signal is predominantly cytoplasmic with a perinuclear distribution pattern, and nuclear staining is minimal under the conditions tested. Heat induced epitope retrieval was performed by steaming sections in citrate buffer, pH 6.0, for 20 minutes prior to immunostaining.

IHC testing of FFPE human intestinal cancer tissue with UPF1 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE mouse intestine with UPF1 antibody. HIER: HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE rat intestine with UPF1 antibody. HIER: HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

Western blot testing of human 1) HeLa, 2) Raji, 3) HepG2, 4) SK-OV-3, 5) PC-3, 6) HEK293, 7) rat RH35 and 8) mouse HEPA1-6 cell lysate with UPF1 antibody. Predicted molecular weight ~124 kDa, but routinely observed at 130-140 kDa.

Flow cytometry testing of human PC-3 cells with UPF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UPF1 antibody.