UGT1A1 Antibody / Drug Metabolism Enzyme Antibody (RQ4094)

UGT1A1 Antibody / Drug Metabolism Enzyme Antibody Human Liver Cancer IHC. Immunohistochemical staining of FFPE human liver cancer tissue using UGT1A1 Antibody / Drug Metabolism Enzyme Antibody. UGT1A1 expression is detected predominantly within the cytoplasm of tumor cells, consistent with its role as a Phase II metabolic enzyme that catalyzes bilirubin conjugation and glucuronidation of endogenous compounds, xenobiotics, and therapeutic drugs. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

UGT1A1 Antibody / Drug Metabolism Enzyme Antibody CACO-2 Immunofluorescence. Immunofluorescent staining of CACO-2 cells using UGT1A1 Antibody / Drug Metabolism Enzyme Antibody (green) with DAPI nuclear counterstain (blue). Enzymatic antigen retrieval was performed for 15 minutes prior to staining. Cells were blocked with 10% goat serum and incubated with the primary antibody (5 ug/ml) overnight at 4°C, followed by a Fluoro488-conjugated goat anti-rabbit IgG secondary antibody. UGT1A1 displays predominantly cytoplasmic staining, consistent with its localization as a Phase II metabolic enzyme involved in bilirubin conjugation, glucuronidation, and the detoxification of endogenous compounds and therapeutic drugs.

UGT1A1 Antibody / Drug Metabolism Enzyme Antibody Human, Mouse and Rat WB. Western blot analysis of 1) human RT4, 2) human CACO-2, 3) rat liver, and 4) mouse liver lysates using UGT1A1 Antibody / Drug Metabolism Enzyme Antibody. A specific immunoreactive band is detected at approximately 55 kDa in all tested samples, consistent with UGT1A1 expression. The predicted molecular weight of UGT1A1 is approximately 60 kDa. These results demonstrate reliable detection of UGT1A1, a major Phase II metabolic enzyme responsible for bilirubin conjugation, glucuronidation, and the detoxification of endogenous compounds, xenobiotics, and therapeutic drugs.

UGT1A1 Antibody / Drug Metabolism Enzyme Antibody HepG2 Flow Cytometry. Flow cytometric analysis of fixed and permeabilized human HepG2 cells using UGT1A1 Antibody / Drug Metabolism Enzyme Antibody at 1 ug/10^6 cells. Cells were fixed with 4% paraformaldehyde, permeabilized, and blocked with 10% normal goat serum prior to staining. Red: unstained cells. Green: rabbit IgG isotype control. Blue: UGT1A1 antibody. The distinct rightward shift of the UGT1A1 antibody signal relative to the isotype control demonstrates specific intracellular detection of UGT1A1 in HepG2 cells.