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Home >> Antibodies >> Ufm1-conjugating enzyme 1 Antibody / Ufc1

Ufm1-conjugating enzyme 1 Antibody / Ufc1 (F54767)

  Catalog No Formulation Size Price (USD)  
Image F54767-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54767-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human bladder carcinoma tissue with Ufm1-conjugating enzyme 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Ufm1-conjugating enzyme 1 antibody.
Western blot testing of mouse kidney tissue lysate with Ufm1-conjugating enzyme 1 antibody. Predicted molecular weight ~19 kDa.
Flow cytometry testing of human HEK293 cells with Ufm1-conjugating enzyme 1 antibody; Blue=isotype control, Green= Ufm1-conjugating enzyme 1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q9Y3C8
Applications Western blot : 1:500-1:1000
Immunohistochemistry (FFPE) : 1:10-1:50
Flow cytometry : 1:10-1:50 (1x10e6 cells)
Limitations This Ufm1-conjugating enzyme 1 antibody is available for research use only.
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Description

Various ubiquitin-like (UBL) post-translational modifiers contribute critically to the protein degradation pathway . These UBLs are covalently linked to most target proteins through an enzymatic cascade analogous to ubiquitylation, consisting of E1 (activating), E2 (conjugating), and E3 (ligating) enzymes. Ufc1 Belongs to the ubiquitin-conjugating enzyme family, and is an E2-like enzyme which forms an intermediate with UFM1, a novel ubiquitin-fold modifier, via thioester linkage.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Ufm1-conjugating enzyme 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminal region of the human protein was used as the immunogen for the Ufm1-conjugating enzyme 1 antibody.

Storage

Aliquot the Ufm1-conjugating enzyme 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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